Song Wentao, Granville Anthony M
Centre for Advanced Macromolecular Design, School of Chemical Engineering, The University of New South Wales, Sydney, NSW, Australia, 2052.
Methods Mol Biol. 2016;1367:123-35. doi: 10.1007/978-1-4939-3130-9_10.
Distillation-precipitation polymerization of divinylbenzene was applied to obtain uniform-sized polymeric microspheres. The microspheres were then modified with polypentafluorostyrene chains utilizing surface-initiated atom transfer radical polymerization techniques. The hydrophobic fluoropolymer-coated microsphere was then converted to a hydrophilic biopolymer by performing thiol-halogen click chemistry between polypentafluorostyrene and 1-thio-β-D-glucose sodium salt. The semi-fluorinated glycopolymer showed good binding ability with Concanavalin A as determined by confocal microscopy and turbidity experiments.
采用二乙烯基苯的蒸馏沉淀聚合法制备尺寸均匀的聚合物微球。然后利用表面引发的原子转移自由基聚合技术，用聚五氟苯乙烯链对微球进行改性。通过在聚五氟苯乙烯和1-硫代-β-D-葡萄糖钠盐之间进行硫醇-卤素点击化学，将疏水的含氟聚合物包覆微球转化为亲水生物聚合物。通过共聚焦显微镜和浊度实验测定，该半氟化糖聚合物对伴刀豆球蛋白A具有良好的结合能力。
