Krannig Kai-Steffen, Schlaad Helmut
Department of Colloid Chemistry, Max Planck Institute of Colloids and Interfaces, Research Campus Golm, 14424, Potsdam, Germany.
Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.
Methods Mol Biol. 2016;1367:61-7. doi: 10.1007/978-1-4939-3130-9_6.
Glycopolypeptides are prepared either by the polymerization of glycosylated amino acid N-carboxyanhydrides (NCAs) or by the post-polymerization functionalization of polypeptides with suitable functional groups. Here we present a method for the in-situ functionalization and (co-) polymerization of allylglycine N-carboxyanhydride in a facile one-pot procedure, combining radical thiol-ene photochemistry and nucleophilic ring-opening polymerization techniques, to yield well-defined heterofunctional glycopolypeptides.
糖多肽可通过糖基化氨基酸N-羧基酸酐（NCA）的聚合反应制备，也可通过多肽与合适官能团的聚合后官能化反应制备。在此，我们展示了一种原位官能化和烯丙基甘氨酸N-羧基酸酐一锅法（共）聚合的方法，该方法结合了自由基硫醇-烯光化学和亲核开环聚合技术，以生成结构明确的杂功能糖多肽。
