Semsarilar Mona, Canton Irene, Ladmiral Vincent
IEM (Institut Européen des Membranes), UMR 5635 (CNRS-ENSCM-UM), Université de Montpellier, CC047, Place E. Bataillon, 34095, Montpellier, France.
The Centre for Stem Cell Biology (CSCB), The University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.
Methods Mol Biol. 2016;1367:89-108. doi: 10.1007/978-1-4939-3130-9_8.
Glycopolymer-based nanostructures are invaluable tools to both study biological phenomena and to design future targeted drug delivery systems. Polymerization-induced self-assembly, especially RAFT aqueous dispersion polymerization is a unique method to prepare such polymer nanostructures, as it enables the preparation of very-well-defined morphologies at very high concentrations. Here we describe the implementation of PISA to the synthesis of galactosylated spheres, wormlike micelles and vesicles, and the preliminary results of cell toxicity, cell uptake, and cargo delivering capacity of galactose-decorated vesicles.
基于糖聚合物的纳米结构是研究生物现象和设计未来靶向给药系统的宝贵工具。聚合诱导自组装，特别是可逆加成-断裂链转移（RAFT）水分散聚合是制备此类聚合物纳米结构的独特方法，因为它能够在非常高的浓度下制备出定义明确的形态。在这里，我们描述了将聚合诱导自组装用于合成半乳糖化球体、蠕虫状胶束和囊泡，以及半乳糖修饰囊泡的细胞毒性、细胞摄取和载药能力的初步结果。
