生物素化糖功能化量子点的合成方法：用于生物医学应用的新型荧光探针

Synthetic Approach to Biotinylated Glyco-Functionalized Quantum Dots: A New Fluorescent Probes for Biomedical Applications.

作者信息

Adokoh Christian K, Darkwa James, Narain Ravin

机构信息

Department of Chemical and Materials Engineering, University of Alberta, 9107-116 Street, Edmonton, AB, T6G 2V4, Canada.

Department of Chemistry, University of Johannesburg, 524, Auckland Park, 2006, South Africa.

出版信息

Methods Mol Biol. 2016;1367:109-21. doi: 10.1007/978-1-4939-3130-9_9.

DOI:10.1007/978-1-4939-3130-9_9

PMID:26537468

Abstract

Technological advances that allow deeper penetration in live tissues, such as the development of confocal and the generation of ever-new fluorophores that facilitate bright labeling of cells and tissue components have made imaging of vertebrate model organisms efficient and highly informative. Recently, high luminescence, single-excitation narrow emission, low photo bleaching properties, and low toxicity of high-quality water-soluble QDs have attracted attention for in vivo labeling/imaging of cells. Herein we describe a synthetic approach to biotinylated glycopolymer functionalized quantum dots, with special emphasis on the development of high-quality water-soluble and bioactive QDs with low toxicity for fluorescent probes in biomedical applications.

摘要

技术进步使得在活组织中能够进行更深层次的穿透，例如共聚焦技术的发展以及不断涌现的新型荧光团的产生，这些荧光团有助于对细胞和组织成分进行明亮标记，从而使脊椎动物模型生物的成像变得高效且信息丰富。最近，高质量水溶性量子点的高发光性、单激发窄发射、低光漂白特性以及低毒性，使其在细胞的体内标记/成像方面备受关注。在此，我们描述了一种合成生物素化糖聚合物功能化量子点的方法，特别强调开发用于生物医学应用荧光探针的高质量、低毒性水溶性且具有生物活性的量子点。