Liu Wei, Ling Shukuan, Sun Weijia, Liu Tong, Li Yuheng, Zhong Guohui, Zhao Dingsheng, Zhang Pengfei, Song Jinping, Jin Xiaoyan, Xu Zi, Song Hailin, Li Qi, Liu Shujuan, Chai Meng, Dai Qinyi, He Yi, Fan Zhanming, Zhou Yu Jie, Li Yingxian
Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
Sci Rep. 2015 Nov 5;5:16099. doi: 10.1038/srep16099.
The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with the severity of coronary artery calcification (CAC), and testify whether the selected miRNAs could reflect the obstructive coronary artery disease in symptomatic patients. Patients with chest pain and moderated risk for coronary artery disease (CAD) were characterized with coronary artery calcium score (CACS) from cardiac computed tomography (CT). We analyzed plasma miRNA levels of clinical matched 11 CAC (CACS > 100) and 6 non-CAC (CACS = 0) subjects by microarray profile. Microarray analysis identified 34 differentially expressed miRNAs between CAC and non CAC groups. Eight miRNAs (miR-223, miR-3135b, miR-133a-3p, miR-2861, miR-134, miR-191-3p, miR-3679-5p, miR-1229 in CAC patients) were significantly increased in CAC plasma in an independent clinical matched cohort. Four miRNAs (miR-2861, 134, 1229 and 3135b) were correlated with the degree of CAC. Validation test in angiographic cohort showed that miR-134, miR-3135b and miR-2861 were significantly changed in patients with obstructive CAD . We identified three significantly upregulated circulating miRNAs (miR-134, miR-3135b and 2861) correlated with CAC while detected obstructive coronary disease in symptomatic patients.
本研究的目的是寻找与冠状动脉钙化（CAC）严重程度相关的循环微RNA（miRNA），并验证所选的miRNA是否能反映有症状患者的阻塞性冠状动脉疾病。对有胸痛且患冠状动脉疾病（CAD）风险中等的患者进行心脏计算机断层扫描（CT）以测定冠状动脉钙评分（CACS）。我们通过微阵列分析临床匹配的11例CAC（CACS>100）和6例非CAC（CACS = 0）受试者的血浆miRNA水平。微阵列分析确定了CAC组和非CAC组之间34种差异表达的miRNA。在独立的临床匹配队列中，8种miRNA（CAC患者中的miR-223、miR-3135b、miR-133a-3p、miR-2861、miR-134、miR-191-3p、miR-3679-5p、miR-1229）在CAC血浆中显著增加。4种miRNA（miR-2861、134、1229和3135b）与CAC程度相关。血管造影队列中的验证试验表明，在阻塞性CAD患者中miR-134、miR-3135b和miR-2861有显著变化。我们鉴定出三种显著上调的循环miRNA（miR-134、miR-3135b和2861）与CAC相关，同时在有症状患者中检测到阻塞性冠状动脉疾病。
