Wang Hua, Chen Feng, Tong Jiabin, Li Yingying, Cai Jianping, Wang Yan, Li Peng, Hao Yichun, Tian Weimeng, Lv You, Chong Jia, Yang Jiefu
Department of Cardiology, Beijing Hospital, National Center of Ger ontology, 100730, Beijing, China.
Cardiol J. 2017;24(1):65-73. doi: 10.5603/CJ.a2016.0097. Epub 2016 Oct 17.
Circulating microRNAs (miRNAs) potentially carry disease-specific information. In the current study, we aim to characterize the miRNA signature in plasma from dilated cardiomyopathy (DCM) patients and assess the possible correlation between expression levels of circulating miRNAs and symptom severity in DCM patients.
Using microarray-based miRNA expression profiling, we compared the miRNA expression levels in plasma samples from 4 DCM patients and 3 healthy controls. The expression levels of selected differentially expressed, upregulated miRNAs (miR-3135b, miR-3908 and miR-5571-5p) were validated independently in plasma samples from 19 DCM patients and 20 controls.
We observed that plasma miR-3135b (p < 0.001), miR-3908 (p < 0.001) and miR-5571-5p (p < 0.001) were significantly upregulated in DCM patients. The area under receiver operating characteristic (ROC) curves for the 3 miRNAs ranged from 0.83 to 1.00. Moreover, miR-5571-5p levels in plasma were significantly upregulated with severe New York Heart Association (NYHA) classification (p < 0.05).
The circulating miRNAs (miR-3135b, miR-3908 and miR-5571-5p) have potential as diagnostic biomarkers for DCM. Additionally, miR-5571-5p correlated with NYHA classification.
循环微RNA（miRNA）可能携带疾病特异性信息。在本研究中，我们旨在表征扩张型心肌病（DCM）患者血浆中的miRNA特征，并评估循环miRNA表达水平与DCM患者症状严重程度之间的可能相关性。
使用基于微阵列的miRNA表达谱分析，我们比较了4例DCM患者和3例健康对照者血浆样本中的miRNA表达水平。在19例DCM患者和20例对照者的血浆样本中独立验证了所选差异表达、上调的miRNA（miR-3135b、miR-3908和miR-5571-5p）的表达水平。
我们观察到DCM患者血浆中的miR-3135b（p < 0.001）、miR-3908（p < 0.001）和miR-5571-5p（p < 0.001）显著上调。这3种miRNA的受试者工作特征（ROC）曲线下面积在0.83至1.00之间。此外，随着纽约心脏协会（NYHA）分级严重程度增加，血浆中miR-5571-5p水平显著上调（p < 0.05）。
循环miRNA（miR-3135b、miR-3908和miR-5571-5p）有潜力作为DCM的诊断生物标志物。此外，miR-5571-5p与NYHA分级相关。
