Espinoza-Mellado María del Rosario, Reyes-Picaso Carolina, Garcés-Pérez Miriam S, Jardón-Serrano Cynthia V, López-Villegas Edgar O, Giono-Cerezo Silvia
Central de instrumentación de microscopía, Departamento de Investigación, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB- IPN), Prolongación de Carpio y Plan de Ayala, 11340, Mexico, DF, Mexico.
Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB- IPN), Prolongación de Carpio y Plan de Ayala, 11340, Mexico, DF, Mexico.
Arch Microbiol. 2016 Mar;198(2):199-204. doi: 10.1007/s00203-015-1167-3. Epub 2015 Nov 3.
The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.
MAP-LC3系统调节自噬相关液泡的细胞内形成。这些液泡含有LC3蛋白；因此，它已被用作鉴定自噬体的标志物。我们研究的目的是调查流感嗜血杆菌菌株及其上清液是否能激活人喉癌细胞系（HEp-2）中的自噬。我们证明，特别是不可分型流感嗜血杆菌（NTHi）49766及其含有<50 kDa蛋白质的上清液可诱导LC3B-II蛋白的更高表达。超微结构研究显示具有双膜和/或内部膜物质的液泡，其特征与自噬液泡相似。总之，我们的研究结果表明，流感嗜血杆菌菌株及其上清液会引发自噬过程。
