处于绿色吸收光产物状态的蓝细菌视紫红质NpR6012g4的1H、13C和15N化学位移归属
1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state.
作者信息
Lim Sunghyuk, Yu Qinhong, Rockwell Nathan C, Martin Shelley S, Lagarias J Clark, Ames James B
机构信息
Department of Chemistry, University of California, Davis, CA, 95616, USA.
Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
出版信息
Biomol NMR Assign. 2016 Apr;10(1):157-61. doi: 10.1007/s12104-015-9657-4. Epub 2015 Nov 4.
Abstract
Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins with a tetrapyrrole (bilin) chromophore that belong to the phytochrome superfamily. Like phytochromes, CBCRs photoconvert between two photostates with distinct spectral properties. NpR6012g4 from Nostoc punctiforme is a model system for widespread CBCRs with conserved red/green photocycles. Atomic-level structural information for the photoproduct state in this subfamily is not known. Here, we report NMR backbone chemical shift assignments of the light-activated state of NpR6012g4 (BMRB no. 26577) as a first step toward determining its atomic resolution structure.
摘要
蓝细菌色素（CBCRs）是蓝细菌中的光感受蛋白，带有属于植物色素超家族的四吡咯（胆色素）发色团。与植物色素一样，CBCRs在具有不同光谱特性的两种光状态之间进行光转换。来自点状念珠藻的NpR6012g4是具有保守红/绿光循环的广泛存在的CBCRs的模型系统。该亚家族中光产物状态的原子水平结构信息尚不清楚。在这里，我们报告了NpR6012g4光激活状态的核磁共振主链化学位移归属（生物磁共振银行编号26577），作为确定其原子分辨率结构的第一步。
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