Rodrigues Carlos J C, de Carvalho Carla C C R
iBB - Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
iBB - Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
FEMS Microbiol Ecol. 2015 Dec;91(12). doi: 10.1093/femsec/fiv135. Epub 2015 Nov 3.
Several parameters are involved in bacterial adhesion and biofilm formation including surface type, medium composition and cellular surface hydrophobicty. When the cells are placed inside tubes, parameters such as oxygen availability should also influence cell adhesion. To understand which cellular lipids are involved in the molecular events of biofilm formation in Rhodococcus erythropolis, cell adhesion was promoted on different metallic and non-metallic surfaces immersed in culture media. These cells were able to modulate the fatty acid composition of the cell membrane in response to both the surface to which they adhered and the growth medium used. To assess the response of the cells to both surfaces and operational conditions, biofilms were also promoted inside a reactor built with five different types of tubes and with medium recirculation. The biofilm biomass could be directly related not to the hydrophobicity of the tubes used but to the oxygen permeability of the tubes. Besides this, cell age influenced the adhesion of the R. erythropolis cells to the tubes. Principal component analysis showed that the lipid composition of the cells could separate cells attached to metallic from those on non-metallic surfaces in the plane formed by PC1 and PC2, and influence biofilm biomass.
细菌黏附和生物膜形成涉及多个参数，包括表面类型、培养基成分和细胞表面疏水性。当细胞置于试管中时，诸如氧气供应等参数也会影响细胞黏附。为了解哪些细胞脂质参与了红平红球菌生物膜形成的分子事件，在浸入培养基中的不同金属和非金属表面上促进细胞黏附。这些细胞能够根据它们所黏附的表面和所用的生长培养基来调节细胞膜的脂肪酸组成。为了评估细胞对表面和操作条件的反应，还在一个由五种不同类型的试管构建并进行培养基再循环的反应器内促进生物膜形成。生物膜生物量可能与所用试管的疏水性没有直接关系，而是与试管的氧气渗透性有关。除此之外，细胞年龄影响红平红球菌细胞对试管的黏附。主成分分析表明，细胞的脂质组成能够在由主成分1和主成分2形成的平面上区分附着在金属表面的细胞和附着在非金属表面的细胞，并影响生物膜生物量。
