de Carvalho Carla C C R, Inácio Patrick L, Miranda Rosa M, Santos Telmo G
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
UNIDEMI, Department of Mechanical and Industrial Engineering, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal.
Materials (Basel). 2017 Jul 12;10(7):788. doi: 10.3390/ma10070788.
In an increasingly miniaturised technological world, non-destructive testing (NDT) methodologies able to detect defects at the micro scale are necessary to prevent failures. Although several existing methods allow the detection of defects at that scale, their application may be hindered by the small size of the samples to examine. In this study, the application of bacterial cells to help the detection of fissures, cracks, and voids on the surface of metals is proposed. The application of magnetic and electric fields after deposition of the cells ensured the distribution of the cells over the entire surfaces and helped the penetration of the cells inside the defects. The use of fluorophores to stain the cells allowed their visualisation and the identification of the defects. Furthermore, the size and zeta potential of the cells and their production of siderophores and biosurfactants could be influenced to detect smaller defects. Micro and nano surface defects made in aluminium, steel, and copper alloys could be readily identified by two strains and cells.
在日益小型化的技术世界中，能够在微观尺度上检测缺陷的无损检测（NDT）方法对于预防故障是必不可少的。尽管现有的几种方法能够检测该尺度下的缺陷，但其应用可能会因待检测样品尺寸过小而受到阻碍。在本研究中，提出应用细菌细胞来帮助检测金属表面的裂缝、裂纹和空隙。细胞沉积后施加磁场和电场可确保细胞在整个表面分布，并有助于细胞渗入缺陷内部。使用荧光团对细胞进行染色可实现其可视化以及缺陷的识别。此外，可对细胞的大小、zeta电位及其铁载体和生物表面活性剂的产生进行调控，以检测更小的缺陷。两种菌株和细胞能够轻松识别铝、钢和铜合金中产生的微米和纳米级表面缺陷。
