Nicholson Wayne L, Park Roy
Department of Microbiology and Cell Science, Space Life Sciences Laboratory, University of Florida, 505 Odyssey Way, Room 201-B, Exploration Park at Kennedy Space Center, Merritt Island, FL 32953, USA
Department of Microbiology and Cell Science, Space Life Sciences Laboratory, University of Florida, 505 Odyssey Way, Room 201-B, Exploration Park at Kennedy Space Center, Merritt Island, FL 32953, USA.
FEMS Microbiol Lett. 2015 Dec;362(24):fnv213. doi: 10.1093/femsle/fnv213. Epub 2015 Nov 3.
Spontaneous rifampicin-resistant (RFM(R)) mutants were isolated from Bacillus subtilis 168 cultivated in the presence or absence of oxygen. By DNA sequencing, the mutations were located within Cluster I of the rpoB gene encoding the β subunit of RNA polymerase. The spectrum of RFM(R) rpoB mutations isolated from B. subtilis cells grown anaerobically differed from aerobically grown cells, not only with respect to the location of mutations within Cluster I but also in the class of mutation observed (transition versus transversion). In the absence of RFM, RFM(R) mutants exhibited poorer growth under anaerobic conditions than did the wild-type strain, indicating their lower fitness in the absence of antibiotic selection.
从在有氧或无氧条件下培养的枯草芽孢杆菌168中分离出自发性耐利福平（RFM(R)）突变体。通过DNA测序，这些突变位于编码RNA聚合酶β亚基的rpoB基因的簇I内。从厌氧生长的枯草芽孢杆菌细胞中分离出的RFM(R) rpoB突变体谱与需氧生长的细胞不同，不仅在簇I内的突变位置方面，而且在观察到的突变类别（转换与颠换）方面。在没有利福平的情况下，RFM(R)突变体在厌氧条件下的生长比野生型菌株差，这表明它们在没有抗生素选择时适应性较低。
