赋予枯草芽孢杆菌利福平抗性的新型rpoB突变：对生长、感受态、芽孢形成和萌发的全局影响
Novel rpoB mutations conferring rifampin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germination.
作者信息
Maughan Heather, Galeano Belinda, Nicholson Wayne L
机构信息
Departments of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA.
出版信息
J Bacteriol. 2004 Apr;186(8):2481-6. doi: 10.1128/JB.186.8.2481-2486.2004.
Abstract
Previously, spontaneous rifampin resistance mutations were isolated in cluster I of the rpoB gene, resulting in amino acid replacements (Q469R, H482R, H482Y, or S487L) in the Bacillus subtilis RNA polymerase beta subunit (W. L. Nicholson and H. Maughan, J. Bacteriol. 184:4936-4940, 2002). In this study, each amino acid change in the beta subunit was observed to result in its own unique spectrum of effects on growth and various developmental events, including sporulation, germination, and competence for transformation. The results thus establish the important role played by the RNA polymerase beta subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis.
摘要
此前，在rpoB基因的第一簇中分离出了自发的利福平抗性突变，导致枯草芽孢杆菌RNA聚合酶β亚基出现氨基酸替换（Q469R、H482R、H482Y或S487L）（W. L. 尼科尔森和H. 莫恩，《细菌学杂志》184:4936 - 4940，2002年）。在本研究中，观察到β亚基中的每个氨基酸变化都会对生长以及包括芽孢形成、萌发和转化能力在内的各种发育事件产生其独特的影响谱。因此，这些结果确立了RNA聚合酶β亚基不仅在转录的催化方面，而且在枯草芽孢杆菌主要发育事件的调控中所起的重要作用。
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