Perera Asanthi, Clarke Charles M, Dykes Gary A, Fegan Narelle
School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
CSIRO, Food and Nutrition Flagship, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Biomed Res Int. 2015;2015:382403. doi: 10.1155/2015/382403. Epub 2015 Oct 11.
Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx 2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx 1a, stx 2a, stx 2c, and ehxA and the other carrying stx 1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.
志贺毒素大肠杆菌（STEC）O157及其他几种非O157 STEC血清型是全球人类严重疾病的病原体。本研究旨在对马来西亚反刍动物中的STEC O157及非O157血清型O26、O103、O111、O121、O45和O145进行特征分析。从马来西亚半岛的6个不同农场共采集了136份反刍动物粪便样本。使用免疫磁珠分离大肠杆菌O157和非O157血清型，同时采用PCR对STEC分离株进行检测和分型。从6份（4%）粪便样本中分离出STEC O157:H7，所有分离株均携带stx 2c、eaeA-γ1和ehxA。从2份（1.5%）粪便样本中分离出非O157 STEC，一份分离株携带stx 1a、stx 2a、stx 2c和ehxA，另一份仅携带stx 1a。本研究中一小部分反刍动物体内存在STEC O157和非O157及其毒力特征表明，它们可能对公共卫生影响有限。
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