Scholz P, Haring V, Wittmann-Liebold B, Ashman K, Bagdasarian M, Scherzinger E
Max-Planck Institute for Molecular Genetics, Berlin, F.R.G.
Gene. 1989 Feb 20;75(2):271-88. doi: 10.1016/0378-1119(89)90273-4.
We present the complete nucleotide sequence of RSF1010, a naturally occurring broad-host-range plasmid belonging to the Escherichia coli incompatibility group Q and encoding resistance to streptomycin and sulfonamides. A molecule of RSF1010 DNA consists of 8684 bp and has a G + C content of 61%. Analysis of the distribution of translation start and stop codons in the sequence has revealed the existence of more than 40 open reading frames potentially capable of encoding polypeptides of 60 or more amino acids. To date, products of eleven such potential RSF1010 genes have been identified through the application of controlled expression vector systems, and for eight of them, the reading frame has been confirmed by N- and/or C-terminal amino acid sequence determinations on the purified proteins. The sequencing results are discussed in relation to the systems of replication, host range, conjugal mobilization and antibiotic resistance determinants associated with the RSF1010 plasmid.
我们公布了RSF1010的完整核苷酸序列，它是一种天然存在的广宿主范围质粒，属于大肠杆菌不相容群Q，编码对链霉素和磺胺类药物的抗性。RSF1010 DNA分子由8684个碱基对组成，G + C含量为61%。对序列中翻译起始和终止密码子分布的分析表明，存在40多个可能编码60个或更多氨基酸多肽的开放阅读框。迄今为止，通过应用可控表达载体系统已鉴定出11个此类潜在RSF1010基因的产物，其中8个基因的阅读框已通过对纯化蛋白质的N端和/或C端氨基酸序列测定得到证实。结合与RSF1010质粒相关的复制、宿主范围、接合转移和抗生素抗性决定因素系统对测序结果进行了讨论。
