Nymo Ingebjørg Helena, Beckmen Kimberlee, Godfroid Jacques
1 UiT-The Arctic University of Norway, Research Group for Arctic Infection Biology, Postboks 6050 Langnes, N-9010 Tromsø, Norway.
2 Alaska Department of Fish and Game, 1300 College Road, Fairbanks, Alaska 99701-1551, USA.
J Wildl Dis. 2016 Jan;52(1):96-9. doi: 10.7589/2015-04-100. Epub 2015 Nov 5.
We used an indirect enzyme-linked immunosorbent assay (iELISA) and the rose bengal test (RBT) to test for anti-Brucella antibodies in moose (Alces alces gigas), muskoxen (Ovibos moschatus), and plains bison (Bison bison bison) from various game management units (GMUs) in Alaska, US, sampled from 1982 to 2010. A portion of the sera had previously been tested with the standard plate test (SPT), the buffered Brucella antigen (BBA) card test, and the card test (CARD). No antibody-positive plains bison were identified. Anti-Brucella antibodies were detected in moose (iELISA, n=4/87; RBT, n=4/87; SPT, n=4/5; BBA, n=4/4) from GMU 23 captured in 1992, 1993, and 1995 and in muskoxen (iELISA, n=4/52; RBT, n=4/52; CARD, n=4/35) from GMUs 26A and 26B captured in 2004, 2006, and 2007. A negative effect of infection on the health of individuals of these species is probable. The presence of antibody-positive animals from 1992 to 2007 suggests presence of brucellae over time. The antibody-positive animals were found in northern Alaska, an area with a historically higher prevalence of Brucella-positive caribou, and a spillover of Brucella suis biovar 4 from caribou may have occurred. Brucella suis biovar 4 causes human brucellosis, and transmission from consumption of moose and muskoxen is possible.
我们采用间接酶联免疫吸附测定法（iELISA）和孟加拉红试验（RBT），对1982年至2010年期间从美国阿拉斯加不同狩猎管理单位（GMU）采集的驼鹿（Alces alces gigas）、麝牛（Ovibos moschatus）和平原野牛（Bison bison bison）进行抗布鲁氏菌抗体检测。部分血清此前已通过标准平板试验（SPT）、缓冲布鲁氏菌抗原（BBA）卡片试验和卡片试验（CARD）进行检测。未发现抗体呈阳性的平原野牛。在1992年、1993年和1995年捕获于GMU 23的驼鹿中检测到抗布鲁氏菌抗体（iELISA，n = 4/87；RBT，n = 4/87；SPT，n = 4/5；BBA，n = 4/4），以及在2004年、2006年和2007年捕获于GMU 26A和26B的麝牛中检测到抗布鲁氏菌抗体（iELISA，n = 4/52；RBT，n = 4/52；CARD，n = 4/35）。感染可能对这些物种个体的健康产生负面影响。1992年至2007年期间存在抗体呈阳性的动物，这表明布鲁氏菌长期存在。抗体呈阳性的动物在阿拉斯加北部被发现，该地区历史上布鲁氏菌阳性驯鹿的患病率较高，可能发生了猪布鲁氏菌生物变种4从驯鹿的溢出。猪布鲁氏菌生物变种4可导致人类布鲁氏菌病，通过食用驼鹿和麝牛可能发生传播。
