Forbes L B, Tessaro S V, Lees W
Agriculture and Agrifood Canada, Health of Animals Laboratory/Saskatoon, Saskatchewan, Canada.
J Wildl Dis. 1996 Jan;32(1):94-104. doi: 10.7589/0090-3558-32.1.94.
Four moose (Alces alces) were inoculated conjunctivally with B. abortus biovar 1 to determine their susceptibility to brucellosis, and to describe the serology, bacteriology, hematology, clinical chemistry, and pathology associated with infection. All moose became infected. Two moose were killed at day 70 post-exposure, one (83F) died acutely at day 85, and one was killed at day 166. None of the moose had clinical signs, except for 83F immediately before death. Infected moose were readily detected serologically by the buffered antigen plate test, Brewer card test, standard tube agglutination test, and complement fixation test as used for brucellosis in cattle. With the exception of samples from 83F taken 24 hours before death, clinical chemistry, and hematology results were stable for all moose, and similar to normal values seen in cattle. Lesions seen in all moose were indicative of endotoxemia, and moose 83F died of acute endotoxic shock. Brucella abortus biovar 1 was isolated from several tissues in all moose, most notably from lymph nodes where counts often exceeded 4 x 10(4) colony forming units per g of tissue. Thus infection with B. abortus will kill moose, and progression of the disease is likely rapid under field conditions. Moose appear to be a dead-end host for brucellosis.
给4头驼鹿（驼鹿属）结膜接种流产布鲁氏菌生物变种1，以确定它们对布鲁氏菌病的易感性，并描述与感染相关的血清学、细菌学、血液学、临床化学和病理学情况。所有驼鹿均被感染。2头驼鹿在接触后第70天被宰杀，1头（83F）在第85天急性死亡，1头在第166天被宰杀。除83F在死前即刻出现临床症状外，其他驼鹿均无临床症状。通过用于牛布鲁氏菌病检测的缓冲抗原平板试验、布鲁尔卡片试验、标准试管凝集试验和补体结合试验，可很容易地从血清学上检测出受感染的驼鹿。除了83F在死前24小时采集的样本外，所有驼鹿的临床化学和血液学结果均稳定，且与牛的正常值相似。在所有驼鹿身上观察到的病变均表明存在内毒素血症，83F死于急性内毒素休克。在所有驼鹿的多个组织中均分离到流产布鲁氏菌生物变种1，最显著的是在淋巴结中，每克组织中的菌落形成单位计数常常超过4×10⁴。因此，感染流产布鲁氏菌会导致驼鹿死亡，在野外条件下疾病进展可能很快。驼鹿似乎是布鲁氏菌病的终末宿主。
