Tuttolomondo Teresa, Martinelli Federico, Mariotti Lorenzo, Leto Claudio, Maggio Antonella, La Bella Salvatore
a Dipartimento di Scienze Agrarie e Forestali , Università degli Studi di Palermo, viale delle scienze Ed. 4 , Palermo , Italy.
b Department of Sustainable Agriculture, Biodiversity and Food Security , I.E.M.E.S.T. Istituto Euro Mediterraneo di Scienza e Tecnologia , Palermo , Italy.
Nat Prod Res. 2016;30(9):1103-7. doi: 10.1080/14786419.2015.1104672. Epub 2015 Nov 5.
Although Origanum vulgare (L.) has been deeply analysed at phytochemical level, poor knowledge is available regarding non-volatile compounds such as lipids. The aim of this work was to characterise five wild Sicilian Origanum ecotypes from an agronomic, metabolomic and lipidomic perspective. Serradifalco presented higher dry weight and inflorescences/plant than the others while Favara had a significantly higher number of branches per plant and more extensive flowered stratum. Metabolomic analysis, performed with LC-MS-TOF, allowed a preliminary characterisation of the non-volatile metabolome of the five oregano ecotypes Origanum vulgare ssp. hirtum. Twenty-five metabolites were identified belonging to organic acids, amino acids, lysophosphatidylcholines, carnithines, nucleic bases and lysophosphatidylethanolamines. Lipidomic analysis identified 115 polar plant membrane glycerolipid species. Thirteen of them were differentially present in the two chosen ecotypes. The role of these metabolites in plant physiology from a qualitative and pharmacological point of view was discussed.
尽管牛至（Origanum vulgare (L.)）已在植物化学层面进行了深入分析，但对于脂质等非挥发性化合物的了解却很少。这项工作的目的是从农艺学、代谢组学和脂质组学角度对西西里岛的五种野生牛至生态型进行表征。塞拉迪法尔科（Serradifalco）的干重和每株花序数高于其他生态型，而法瓦拉（Favara）每株的枝条数显著更多，开花层更广泛。采用液相色谱 - 质谱 - 飞行时间质谱（LC-MS-TOF）进行的代谢组学分析，对五种牛至生态型牛至（Origanum vulgare ssp. hirtum）的非挥发性代谢组进行了初步表征。鉴定出25种代谢物，属于有机酸、氨基酸、溶血磷脂酰胆碱、肉碱、核苷酸碱基和溶血磷脂酰乙醇胺。脂质组学分析鉴定出115种极性植物膜甘油脂质物种。其中13种在所选的两种生态型中差异存在。从定性和药理学角度讨论了这些代谢物在植物生理学中的作用。
