Gensberger-Reigl Sabrina, Huppert Jochen, Pischetsrieder Monika
Food Chemistry Unit, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Schuhstr. 19, 91052 Erlangen, Germany.
Fresenius Medical Care Deutschland GmbH, Frankfurter Str. 6-8, 66606 St. Wendel, Germany.
J Pharm Biomed Anal. 2016 Jan 25;118:132-138. doi: 10.1016/j.jpba.2015.10.022. Epub 2015 Oct 23.
During heat sterilization of peritoneal dialysis (PD) fluids, the glucose component is partially degraded. The formed glucose degradation products impair biocompatibility and limit the long-term application of PD fluids. As an alternative to glucose, icodextrin, a polyglucose, is used as osmotic agent in PD fluids. After targeted screening for reactive carbonyl compounds, NMR- and MS-analyses very recently revealed 4-deoxyglucosone (4-DG), 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxypentosone (3,4-DDPS), and 5-hydroxymethylfurfural (5-HMF) as main polyglucose degradation products (pGDPs) in icodextrin-based PD fluids. Now, the present study established and validated a UHPLC method with DAD as well as a UHPLC-MS/MS method for the first-time quantification of those five major pGDPs in commercial icodextrin PD fluids after derivatization with o-phenylenediamine. Thus, 4-DG was identified to be the main degradation product (in concentrations up to 20 μM). In contrast to the values measured in glucose-based products, the concentration of 3-DGal (≤ 16 μM) was higher than the concentration of 3-DG (≤ 7 μM) indicating different reaction pathways starting from polyglucose compared to glucose. The compounds 3,4-DDPS and 5-HMF were present in minor quantities (≤ 0.3 μM each).
在腹膜透析（PD）液的热灭菌过程中，葡萄糖成分会部分降解。所形成的葡萄糖降解产物会损害生物相容性，并限制PD液的长期应用。作为葡萄糖的替代品，异麦芽糖糊精（一种聚葡萄糖）被用作PD液中的渗透剂。在针对性筛选反应性羰基化合物后，核磁共振（NMR）和质谱（MS）分析最近揭示，在基于异麦芽糖糊精的PD液中，4-脱氧葡萄糖酮（4-DG）、3-脱氧葡萄糖酮（3-DG）、3-脱氧半乳糖酮（3-DGal）、3,4-二脱氧戊糖酮（3,4-DDPS）和5-羟甲基糠醛（5-HMF）是主要的聚葡萄糖降解产物（pGDPs）。现在，本研究首次建立并验证了一种采用二极管阵列检测器（DAD）的超高效液相色谱（UHPLC）方法以及一种UHPLC-MS/MS方法，用于在邻苯二胺衍生化后对市售异麦芽糖糊精PD液中的这五种主要pGDPs进行定量分析。因此，4-DG被确定为主要降解产物（浓度高达20 μM）。与基于葡萄糖的产品中测得的值相比，3-DGal（≤ 16 μM）的浓度高于3-DG（≤ 7 μM）的浓度，这表明与葡萄糖相比，从聚葡萄糖开始的反应途径不同。化合物3,4-DDPS和5-HMF的含量较少（各自≤ 0.3 μM）。
