Uarrota Virgílio Gavicho, Maraschin Marcelo
Postgraduate Program in Biotechnology and Biosciences, Plant Morphogenesis and Biochemistry Laboratory, Biological Science Center, Federal University of Santa Catarina, Rodovia Admar Gonzaga 1346, Florianópolis, Santa Catarina, CEP 88.034-000, Brazil.
BMC Res Notes. 2015 Nov 5;8:648. doi: 10.1186/s13104-015-1580-3.
Under postharvest physiological deterioration cassava root tubers alter the expression of biosynthetic pathways of certain primary and secondary metabolites, as well as the activity of some scavenging enzymes. Therefore, in this study we hypothesized that cassava cultivars differ as to their physiological responses to deterioration and their biochemical profiles can be an indicative of the tolerance or susceptibility to deterioration.
The results corroborate the working hypothesis, revealing that high Levels of phenolic acids, scopoletin, carotenoids, proteins, and augmented activities of guaiacol peroxidase and hydrogen peroxide in non-stored cassava roots can be used as potential biomarkers of cassava deterioration.
Cassava physiological deterioration depends on cultivar and many compounds are up and downregulated during storage time. Secondary metabolites, enzymes, scopoletin, scavenging reactive oxygen species, and acidic polysaccharides are activated as responses to the physiological stress induced in root tubers.
在采后生理劣变过程中，木薯块根会改变某些初级和次级代谢产物生物合成途径的表达，以及一些清除酶的活性。因此，在本研究中，我们假设木薯品种对劣变的生理反应存在差异，其生化特征可作为耐劣性或易感性的指标。
结果证实了该工作假设，表明未储存木薯根中高水平的酚酸、东莨菪亭、类胡萝卜素、蛋白质以及愈创木酚过氧化物酶和过氧化氢活性的增强可作为木薯劣变的潜在生物标志物。
木薯生理劣变取决于品种，且在储存期间许多化合物的表达会上调或下调。次级代谢产物、酶、东莨菪亭、清除活性氧以及酸性多糖会作为对块根中诱导的生理胁迫的反应而被激活。
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