紫小麦中花色苷及其他植物化学物质的测定
Measurement of anthocyanins and other phytochemicals in purple wheat.
作者信息
Hosseinian Farah S, Li Wende, Beta Trust
机构信息
Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2; Richardson Centre for Functional Foods and Nutraceuticals, Smartpark, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
出版信息
Food Chem. 2008 Aug 15;109(4):916-24. doi: 10.1016/j.foodchem.2007.12.083. Epub 2008 Jan 26.
The major anthocyanin composition of normal purple wheat and heat stressed purple wheat were measured using HPLC, LC-MS/MS and the pH differential method. The lignan secoisolariciresinol diglucoside (SDG) and melatonin content were also measured. Total anthocyanin profile of normal purple wheat (491.3mg/kg) was significantly (P<0.05) lower than that of the heat stressed purple wheat (522.7mg/kg). Thirteen major anthocyanins were isolated and cyanidin 3-glucoside was the predominant anthocyanin in purple wheat. Using the pH differential method, the total anthocyanin content of normal (500.6mg/kg) and heat stressed (526.0mg/kg) purple wheat were similar to those observed using HPLC. The SDG content of normal and heat stressed purple wheat were 770 and 520μg/kg, while melatonin content was 4 and 2μg/kg, respectively. The presence of SDG and melatonin in addition to anthocyanins may contribute to the health benefits associated with consumption of coloured cereal grains.
采用高效液相色谱法（HPLC）、液相色谱-串联质谱法（LC-MS/MS）和pH差值法测定了正常紫色小麦和热胁迫紫色小麦中的主要花青素成分。同时还测定了木脂素开环异落叶松脂素二葡萄糖苷（SDG）和褪黑素的含量。正常紫色小麦的总花青素含量（491.3mg/kg）显著低于（P<0.05）热胁迫紫色小麦（522.7mg/kg）。分离出13种主要花青素，矢车菊素3-葡萄糖苷是紫色小麦中的主要花青素。采用pH差值法，正常（500.6mg/kg）和热胁迫（526.0mg/kg）紫色小麦的总花青素含量与HPLC法测得的结果相似。正常和热胁迫紫色小麦的SDG含量分别为770和520μg/kg，而褪黑素含量分别为4和2μg/kg。除花青素外，SDG和褪黑素的存在可能有助于食用有色谷物带来的健康益处。
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