Byeon Hyeokjun, Ha Young-Ran, Lee Sang Joon
Pohang University of Science and Technology, Department of Mechanical Engineering, San 31. Hyoja-dong, Namgu, Pohang 790-784, Republic of Korea.
Pohang University of Science and Technology, Division of Integrative Biosciences and Biotech, San 31. Hyoja-dong, Namgu, Pohang 790-784, Republic of Korea.
J Biomed Opt. 2015 Nov;20(11):115003. doi: 10.1117/1.JBO.20.11.115003.
Malaria parasites induce morphological, biochemical, and mechanical changes in red blood cells (RBCs). Mechanical variations are closely related to the deformability of individual RBCs. The deformation of various RBCs, including healthy and malaria-infected RBCs (iRBCs), can be directly observed through quantitative phase imaging (QPI). The effects of chloroquine treatment on the mechanical property variation of iRBCs were investigated using time-resolved holographic QPI of single live cells on a millisecond time scale. The deformabilities of healthy RBCs, iRBCs, and drug-treated iRBCs were compared, and the effect of chloroquine on iRBC restoration was experimentally examined. The present results are beneficial to elucidate the dynamic characteristics of iRBCs and the effect of the antimalarial drug on iRBCs.
疟原虫会引起红细胞（RBCs）的形态、生化和机械变化。机械变化与单个红细胞的变形能力密切相关。通过定量相成像（QPI）可以直接观察各种红细胞的变形情况，包括健康的和感染疟疾的红细胞（iRBCs）。使用毫秒时间尺度上的单活细胞时间分辨全息QPI研究了氯喹治疗对iRBCs力学性能变化的影响。比较了健康红细胞、iRBCs和药物处理后的iRBCs的变形能力，并通过实验研究了氯喹对iRBCs恢复的影响。目前的结果有助于阐明iRBCs的动态特征以及抗疟药物对iRBCs的作用。
