载脂蛋白E缺乏的大鼠在部分结扎的颈动脉中会形成动脉粥样硬化斑块。
Apolipoprotein E-deficient rats develop atherosclerotic plaques in partially ligated carotid arteries.
作者信息
Wei Shujie, Zhang Yan, Su Linan, He Kecheng, Wang Qiang, Zhang Yunrong, Yang Dachun, Yang Yongjian, Ma Shuangtao
机构信息
Department of Cardiology, Chengdu Military General Hospital, 270 Tianhui Rd., Rongdu Ave., Jinniu, Chengdu, Sichuan 610083, China.
Department of Cardiology, Chengdu Military General Hospital, 270 Tianhui Rd., Rongdu Ave., Jinniu, Chengdu, Sichuan 610083, China.
出版信息
Atherosclerosis. 2015 Dec;243(2):589-92. doi: 10.1016/j.atherosclerosis.2015.10.093. Epub 2015 Oct 24.
BACKGROUND
The goal of this study was to establish an apolipoprotein E-deficient (ApoE(-/-)) rat model.
METHODS
The ApoE(-/-) rat was created by TALEN-mediated gene targeting in the genetic background of Sprague Dawley rat. Six-to eight-week-old male rats were used in the experiments (n = 10 in each group).
RESULTS
After fed with high-cholesterol diet (HCD) for 12 weeks, the ApoE(-/-) rats displayed typical dyslipidemia. In contrast, HCD failed to induce hypercholesterolemia in wild-type rats. However, there was no obvious atherosclerotic lesion in oil red O-stained en face aortas and the aortic root sections in both genetic types of rats. Interestingly, partial ligation caused the formation of plaques consist of lipid and macrophages in carotid arteries from ApoE(-/-) rats, but induced the neointimal hyperplasia in wild-type rats. Additionally, we found that HCD slightly increased the expression of adhesion molecules, while partial ligation dramatically upregulated these molecules.
CONCLUSIONS
The ApoE(-/-) rat is a novel model for dyslipidemia and could also be used in the research of atherosclerosis.
背景
本研究的目的是建立载脂蛋白E缺陷（ApoE(-/-)）大鼠模型。
方法
在Sprague Dawley大鼠的遗传背景下，通过TALEN介导的基因靶向技术创建ApoE(-/-)大鼠。实验使用6至8周龄的雄性大鼠（每组n = 10）。
结果
在高胆固醇饮食（HCD）喂养12周后，ApoE(-/-)大鼠表现出典型的血脂异常。相比之下，HCD未能在野生型大鼠中诱导高胆固醇血症。然而，两种基因类型大鼠的油红O染色主动脉和主动脉根部切片均未出现明显的动脉粥样硬化病变。有趣的是，部分结扎导致ApoE(-/-)大鼠颈动脉中形成由脂质和巨噬细胞组成的斑块，但在野生型大鼠中诱导了内膜增生。此外，我们发现HCD略微增加了黏附分子的表达，而部分结扎则显著上调了这些分子的表达。
结论
ApoE(-/-)大鼠是一种新型的血脂异常模型，也可用于动脉粥样硬化的研究。
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