小鼠骨髓微核试验可用于区分非整倍体诱导剂和断裂剂。
The mouse bone marrow micronucleus assay can be used to distinguish aneugens from clastogens.
作者信息
Vanderkerken K, Vanparys P, Verschaeve L, Kirsch-Volders M
机构信息
Laboratorium voor Antropogenetica, Vrije Universiteit Brussel, Belgium.
出版信息
Mutagenesis. 1989 Jan;4(1):6-11. doi: 10.1093/mutage/4.1.6.
The occurrence of aneuploidy can be measured in several assays. However, none of them have been sufficiently validated. The bone marrow micronucleus test may be considered as a method for aneuploidy detection. In this work, micronuclei were induced by two aneugens, vincristine sulfate (0.1 mg/kg) and nocodazole (80 mg/kg), and two clastogens ethylmethanesulfonate (EMS) (300 mg/kg) and cyclophosphamide (60 mg/kg). Three criteria have been examined in order to distinguish micronuclei induced by aneugens and clastogens: the area of the micronuclei, the percentage of micronuclei with C-band-positive material and the DNA content of the micronuclei. C-band-positive micronuclei were found in 47% of the micronuclei for vincristine sulfate, 58% for nocodazole, 17% for EMS and 20% for cyclophosphamide. Areas of micronuclei showed a significant difference when induced by aneugens or by clastogens. Finally, the DNA content of micronuclei also showed a totally different distribution pattern when comparing the aneugen vincristine sulfate with the clastogen EMS. The three methods analysed could thus all make a difference between micronuclei induced by aneugens and those induced by clastogens.
非整倍体的发生可以通过多种检测方法进行测量。然而，这些方法均未得到充分验证。骨髓微核试验可被视为一种检测非整倍体的方法。在本研究中，用两种非整倍体诱导剂硫酸长春新碱（0.1 mg/kg）和诺考达唑（80 mg/kg），以及两种致断裂剂甲基磺酸乙酯（EMS）（300 mg/kg）和环磷酰胺（60 mg/kg）诱导微核形成。为了区分由非整倍体诱导剂和致断裂剂诱导产生的微核，研究了三个标准：微核的面积、具有C带阳性物质的微核百分比以及微核的DNA含量。硫酸长春新碱诱导产生的微核中，47%为C带阳性微核；诺考达唑诱导产生的微核中，58%为C带阳性微核；EMS诱导产生的微核中，17%为C带阳性微核；环磷酰胺诱导产生的微核中，20%为C带阳性微核。由非整倍体诱导剂或致断裂剂诱导产生的微核，其面积存在显著差异。最后，将非整倍体诱导剂硫酸长春新碱与致断裂剂EMS相比较时，微核的DNA含量也呈现出完全不同的分布模式。因此，所分析的这三种方法均能够区分由非整倍体诱导剂和致断裂剂诱导产生的微核。
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