马肝炎病毒两个系统发育谱系的鉴定及其在巴西的高流行率。
Identification of two phylogenetic lineages of equine hepacivirus and high prevalence in Brazil.
作者信息
Figueiredo Andreza Soriano, Lampe Elisabeth, do Espírito-Santo Márcia Paschoal, Mello Francisco Campello do Amaral, de Almeida Fernando Queiroz, de Lemos Elba Regina Sampaio, Godoi Tatianne Leme Oliveira Santos, Dimache Luana Avila Giorgia, Dos Santos Debora Regina Lopes, Villar Livia Melo
机构信息
Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, RJ, 21040-360 Brazil.
Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, RJ, 21040-360 Brazil.
出版信息
Vet J. 2015 Dec;206(3):414-6. doi: 10.1016/j.tvjl.2015.10.015. Epub 2015 Oct 22.
Non-primate hepacivirus (NPHV), as described in horses, is the virus most genetically related to hepatitis C virus (HCV). Although detected worldwide, limited data on genomic variability and distribution of NPHV are available in Latin America. The aim of this study was to investigate the genetic diversity and prevalence of equine NPHV in Brazil. Thirteen percent of 202 equines from three Brazilian states were positive for NPHV genome by reverse transcriptase PCR. Nucleotide sequences of the partial NS5B genome presented the greatest diversity described to date (25.6%), which is comparable to the upper limit of diversity for HCV subtype classification for the same region. Phylogenetic analysis revealed that Brazilian NPHV sequences along with isolates worldwide form two strongly supported clades (pp = 1.0) suggesting the existence of two distinct lineages.
如在马身上所描述的那样，非灵长类丙型肝炎病毒（NPHV）是与丙型肝炎病毒（HCV）在基因上关系最为密切的病毒。尽管在全球范围内都有检测到NPHV，但拉丁美洲关于NPHV基因组变异性和分布的可用数据有限。本研究的目的是调查巴西马源NPHV的遗传多样性和流行情况。通过逆转录聚合酶链反应，来自巴西三个州的202匹马中有13%的NPHV基因组呈阳性。部分NS5B基因组的核苷酸序列呈现出迄今为止所描述的最大多样性（25.6%），这与同一区域HCV亚型分类的多样性上限相当。系统发育分析表明，巴西的NPHV序列与全球分离株形成了两个得到有力支持的进化枝（后验概率 = 1.0），这表明存在两个不同的谱系。
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