评估一种三重实时荧光定量PCR系统以检测植物病原霉菌链格孢属、镰刀菌属和紫色麦角菌。
Evaluation of a triplex real-time PCR system to detect the plant-pathogenic molds Alternaria spp., Fusarium spp. and C. purpurea.
作者信息
Grube Sabrina, Schönling Jutta, Prange Alexander
机构信息
Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, D-41065 Mönchengladbach, Germany; Institute of Virology and Microbiology, University of Witten/Herdecke, D-58448 Witten, Germany.
GEN-IAL GmbH, D-53842 Troisdorf, Germany.
出版信息
J Microbiol Methods. 2015 Dec;119:180-8. doi: 10.1016/j.mimet.2015.10.017. Epub 2015 Nov 9.
This article describes the development of a triplex real-time PCR system for the simultaneous detection of three major plant-pathogenic mold genera (Alternaria spp., Fusarium spp. and the species Claviceps purpurea). The designed genus-specific primer-probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA.
本文描述了一种用于同时检测三种主要植物病原霉菌属（链格孢属、镰刀菌属和紫麦角菌）的三重实时荧光定量PCR系统的开发。所设计的属特异性引物-探针系统在存在背景DNA的情况下，针对灵敏度、特异性和扩增情况进行了验证。
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