基于探针的实时荧光定量PCR技术检测临床标本中的菌种
Detection of Species in Clinical Specimens by Probe-Based Real-Time PCR.
作者信息
Springer Jan, Walther Grit, Rickerts Volker, Hamprecht Axel, Willinger Birgit, Teschner Daniel, Einsele Hermann, Kurzai Oliver, Loeffler Juergen
机构信息
Department for Internal Medicine II, University Hospital of Wuerzburg, 97080 Wuerzburg, Germany.
National Reference Center for Invasive Fungal Infections (NRZMyk), Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.
出版信息
J Fungi (Basel). 2019 Nov 12;5(4):105. doi: 10.3390/jof5040105.
The mold is a ubiquitous fungus causing plant, animal and human infections. In humans, spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus we analysed several different clinical specimens detecting spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed ( = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of species and its application to clinically relevant specimens.
该霉菌是一种普遍存在的真菌，可引起植物、动物和人类感染。在人类中，[具体菌种名称]是佩戴隐形眼镜患者或局部创伤后眼部感染的主要原因。[具体菌种名称]引起的全身感染主要发生在免疫抑制患者中，并可在人体内扩散。由于对抗真菌药物的高度耐药性，迫切需要快速鉴定病原体。通过使用针对[具体菌种名称]属的基于探针的实时PCR检测方法，我们分析了几种不同的临床标本，检测德国临床样本中常见的[具体菌种名称]。此外，还分析了大量血液学患者的肺液样本（n = 243）。其中，两个样本（0.8%）反复呈阳性，但只有一个通过测序得到证实。对于这例可能的侵袭性真菌病（IFD）病例，培养结果显示[具体菌种名称]呈阳性。在此，我们描述了一种基于探针的快速实时PCR检测方法，用于特异性检测多种[具体菌种名称]的DNA及其在临床相关标本中的应用。
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