Suppr超能文献

定量图像分析作为评估解脂耶氏酵母在不同培养基中双态生长的工具。

Quantitative image analysis as a tool for Yarrowia lipolytica dimorphic growth evaluation in different culture media.

作者信息

Braga A, Mesquita D P, Amaral A L, Ferreira E C, Belo I

机构信息

CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Instituto Politécnico de Coimbra, ISEC, DEQB, Rua Pedro Nunes, Quinta da Nora, 3030-199 Coimbra, Portugal.

出版信息

J Biotechnol. 2016 Jan 10;217:22-30. doi: 10.1016/j.jbiotec.2015.10.023. Epub 2015 Nov 3.

Abstract

Yarrowia lipolytica, a yeast strain with a huge biotechnological potential, capable to produce metabolites such as γ-decalactone, citric acid, intracellular lipids and enzymes, possesses the ability to change its morphology in response to environmental conditions. In the present study, a quantitative image analysis (QIA) procedure was developed for the identification and quantification of Y. lipolytica W29 and MTLY40-2P strains dimorphic growth, cultivated in batch cultures on hydrophilic (glucose and N-acetylglucosamine (GlcNAc) and hydrophobic (olive oil and castor oil) media. The morphological characterization of yeast cells by QIA techniques revealed that hydrophobic carbon sources, namely castor oil, should be preferred for both strains growth in the yeast single cell morphotype. On the other hand, hydrophilic sugars, namely glucose and GlcNAc caused a dimorphic transition growth towards the hyphae morphotype. Experiments for γ-decalactone production with MTLY40-2P strain in two distinct morphotypes (yeast single cells and hyphae cells) were also performed. The obtained results showed the adequacy of the proposed morphology monitoring tool in relation to each morphotype on the aroma production ability. The present work allowed establishing that QIA techniques can be a valuable tool for the identification of the best culture conditions for industrial processes implementation.

摘要

解脂耶氏酵母是一种具有巨大生物技术潜力的酵母菌株,能够产生γ-癸内酯、柠檬酸、细胞内脂质和酶等代谢产物,它具有根据环境条件改变其形态的能力。在本研究中,开发了一种定量图像分析(QIA)程序,用于鉴定和解脂耶氏酵母W29和MTLY40-2P菌株在亲水性(葡萄糖和N-乙酰葡糖胺(GlcNAc))和疏水性(橄榄油和蓖麻油)培养基上分批培养时的二态生长情况并进行定量分析。通过QIA技术对酵母细胞进行形态表征表明,疏水性碳源,即蓖麻油,对于两种菌株以酵母单细胞形态生长而言应是首选。另一方面,亲水性糖,即葡萄糖和GlcNAc会导致向菌丝形态的二态转变生长。还使用MTLY40-2P菌株在两种不同形态(酵母单细胞和菌丝细胞)下进行了γ-癸内酯生产实验。所得结果表明,所提出的形态监测工具对于每种形态的香气产生能力而言是足够的。目前的工作表明,QIA技术可以成为确定工业过程实施最佳培养条件的有价值工具。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验