Celińska Ewelina, Zhou Yongjin J
Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, ul. Wojska Polskiego 48, 60-637 Poznań, Poland.
Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
FEMS Yeast Res. 2025 Jan 30;25. doi: 10.1093/femsyr/foaf023.
Global transcription machinery engineering (gTME) is a strategy for optimizing complex phenotypes in microbes by manipulating transcription factors (TFs) and their downstream transcriptional regulatory networks (TRN). In principle, gTME leads to a focused but comprehensive optimization of a microbe, also enabling the engineering of nonpathway functionalities, like stress resistance, protein expression, or growth rate. A link between a TF and a desired phenotype is to be established for a rationally designed gTME. For use in a high-throughput format with extensive libraries of TRN-engineered clones tested under multiple conditions, well-developed culturing and analytical protocols are needed, to reveal the pleiotropic effects of the TFs. This mini-review summarizes the gTME strategies and TFs described under different contexts in Yarrowia lipolytica. The outcomes of the gTME strategy application are also addressed, demonstrating its effectiveness in engineering complex, industrially relevant traits in Y. lipolytica.
全局转录机器工程（gTME）是一种通过操纵转录因子（TFs）及其下游转录调控网络（TRN）来优化微生物复杂表型的策略。原则上，gTME可实现对微生物的有针对性但全面的优化，还能实现非途径功能的工程改造，如抗逆性、蛋白质表达或生长速率。对于合理设计的gTME，需要在TF与所需表型之间建立联系。为了以高通量形式使用，在多种条件下测试大量TRN工程克隆文库，需要完善的培养和分析方案，以揭示TF的多效性作用。本综述总结了在解脂耶氏酵母不同背景下描述的gTME策略和TFs。还讨论了gTME策略应用的结果，证明了其在解脂耶氏酵母中工程改造复杂的、与工业相关性状方面的有效性。
