Faculty of Agriculture, Food Engineering Department, Cukurova University, Adana, Turkey.
Altinozu Agricultural Sciences Vocational School, Mustafa Kemal University, Antakya, Turkey.
Yeast. 2019 May;36(5):319-327. doi: 10.1002/yea.3389.
Citric acid (CA) productivity by Yarrowia lipolytica dependents on strain type, carbon source, carbon to nitrogen (C/N) molar ratio as well as physicochemical conditions (pH, temperature, oxygen transfer rate, etc.). In the current study, 10 different Y. lipolytica strains were first challenged in shake-flask culture for CA production in a glucose-based media under nitrogen-limited conditions. For the most promising one, strain K57, CA productivity was monitored during culture in batch bioreactor for three initial C/N molar ratio (167, 367, and 567 Cmol/Nmol). The highest CA yield (0.77 g/g glucose), titre (72.3 g/L CA), and productivity (0.04 g/g.h) were found for C/N ratio of 367. However, the highest growth rate was obtained for an initial C/N ratio of 167. From these results, Y. lipolytica strain K57 could be considered to produce CA at higher titre on glucose-based medium in batch bioreactor than others Y. lipolytica strain reported in the literature.
柠檬酸（CA）的产量取决于脂肪假丝酵母的菌株类型、碳源、碳氮（C/N）摩尔比以及物理化学条件（pH、温度、氧传递速率等）。在本研究中，首先在摇瓶培养中用葡萄糖基培养基在氮限制条件下对 10 种不同的脂肪假丝酵母菌株进行 CA 生产能力的挑战。对于最有前途的 K57 菌株，在分批生物反应器中监测了三种初始 C/N 摩尔比（167、367 和 567 Cmol/Nmol）培养过程中的 CA 生产力。当 C/N 比为 367 时，CA 的产率（0.77 g/g 葡萄糖）、浓度（72.3 g/L CA）和生产效率（0.04 g/g.h）最高。然而，当 C/N 比为 167 时，获得了最高的生长速率。根据这些结果，与文献中报道的其他脂肪假丝酵母菌株相比，K57 菌株在分批生物反应器中利用葡萄糖基培养基可以以更高的浓度生产 CA。
