Rajput Nasir Ahmed, Zhang Meixiang, Shen Danyu, Liu Tingli, Zhang Qimeng, Ru Yanyan, Sun Peng, Dou Daolong
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan These authors contributed equally to this work.
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China These authors contributed equally to this work.
Plant Cell Physiol. 2015 Dec;56(12):2423-35. doi: 10.1093/pcp/pcv164. Epub 2015 Nov 6.
The Crinkler (CRN) effector family is produced by oomycete pathogens and may manipulate host physiological and biochemical events inside host cells. Here, PsCRN161 was identified from Phytophthora sojae based on its broad and strong cell death suppression activities. The effector protein contains two predicted nuclear localization signals and localized to nuclei of plant cells, indicating that it may target plant nuclei to modify host cell physiology and function. The chimeric gene GFP:PsCRN161 driven by the Cauliflower mosaic virus (CaMV) 35S promoter was introduced into Nicotiana benthamiana. The four independent PsCRN161-transgenic lines exhibited increased resistance to two oomycete pathogens (P. parasitica and P. capsici) and showed enhanced tolerance to salinity and drought stresses. Digital gene expression profiling analysis showed that defense-related genes, including ABC transporters, Cyt P450 and receptor-like kinases (RLKs), were significantly up-regulated in PsCRN161-transgenic plants compared with GFP (green fluorescent protein) lines, implying that PsCRN161 expression may protect plants from biotic and abiotic stresses by up-regulation of many defense-related genes. The results reveal previously unknown functions of the oomycete effectors, suggesting that the pathogen effectors could be directly used as functional genes for plant molecular breeding for enhancement of tolerance to biotic and abiotic stresses.
卷曲蛋白（CRN）效应蛋白家族由卵菌病原体产生，可能会操控宿主细胞内的生理和生化活动。在此，基于大豆疫霉具有广泛且强大的细胞死亡抑制活性，鉴定出了PsCRN161。该效应蛋白含有两个预测的核定位信号，并定位于植物细胞核，这表明它可能靶向植物细胞核来改变宿主细胞的生理和功能。由花椰菜花叶病毒（CaMV）35S启动子驱动的嵌合基因GFP:PsCRN161被导入本氏烟草。四个独立的PsCRN161转基因株系对两种卵菌病原体（寄生疫霉和辣椒疫霉）表现出增强的抗性，并对盐胁迫和干旱胁迫表现出更强的耐受性。数字基因表达谱分析表明，与绿色荧光蛋白（GFP）株系相比，包括ABC转运蛋白、细胞色素P450和类受体激酶（RLK）在内的防御相关基因在PsCRN161转基因植物中显著上调，这意味着PsCRN161的表达可能通过上调许多防御相关基因来保护植物免受生物和非生物胁迫。这些结果揭示了卵菌效应蛋白以前未知的功能，表明病原体效应蛋白可直接用作植物分子育种的功能基因，以增强对生物和非生物胁迫的耐受性。
