Yan Yang Shu, Zheng Ying, Huang Zhou, Min Wang Xue, Yang Hong
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
Int J Syst Evol Microbiol. 2016 Jan;66(1):518-522. doi: 10.1099/ijsem.0.000743. Epub 2015 Nov 5.
Bacterial strain M19T was isolated from the gut of a wood-feeding termite, Nasutitermes hainanensis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M19T was related to members of the genus Lactococcus, with sequence similarities ranging from 84.8 to 95.5 %. Comparison of housekeeping gene ropB sequences revealed that strain M19T was well separated from Lactococcus fujiensis JCM 16395T and Lactococcus hircilactis 117T. The isolate was Gram-stain-positive, catalase-negative and non-motile. Cells were coccoid or ovoid-shaped, and occurred singly, in pairs or as short chains. Growth of strain M19T occurred at 10-40 °C and at pH 5.0-7.5. The DNA G+C content of strain M19T was 39.6 mol% and the major fatty acids were C16 : 0, cyclo-C19 : 0ω8c, C18 : 1ω9c, summed feature 7 and summed feature 8. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain M19T represents a novel species of the genus Lactococcus, for which the name Lactococcus nasutitermitis sp. nov. is proposed. The type strain is M19T ( = CGMCC 1.15204T = NBRC 111537T).
细菌菌株M19T是从海南歪白蚁肠道中分离得到的。基于16S rRNA基因序列的系统发育分析表明，菌株M19T与乳球菌属的成员相关，序列相似性范围为84.8%至95.5%。看家基因ropB序列比较显示，菌株M19T与富士乳球菌JCM 16395T和嗜奶乳球菌117T有明显区别。该分离株革兰氏染色阳性，过氧化氢酶阴性，无运动性。细胞呈球状或卵形，单个、成对或成短链状存在。菌株M19T在10-40°C和pH 5.0-7.5条件下生长。菌株M19T的DNA G+C含量为39.6 mol%，主要脂肪酸为C16 : 0、环C19 : 0ω8c、C18 : 1ω9c、总和特征7和总和特征8。根据所提供的系统发育、化学分类和表型数据，菌株M19T代表乳球菌属的一个新物种，为此提出新名称海南歪白蚁乳球菌（Lactococcus nasutitermitis sp. nov.）。模式菌株为M19T（= CGMCC 1.15204T = NBRC 111537T）。
