Meucci Aurora, Zago Miriam, Rossetti Lia, Fornasari Maria Emanuela, Bonvini Barbara, Tidona Flavio, Povolo Milena, Contarini Giovanna, Carminati Domenico, Giraffa Giorgio
Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie (CRA-FLC), 26900 Lodi, Italy.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2091-2096. doi: 10.1099/ijs.0.000225. Epub 2015 Apr 1.
Two strains of lactic acid bacteria, designated 117(T) and 4195(T), were isolated from goat milk in Valtellina, Italy and from cow milk in Valle Trompia, Italy, respectively, and characterized taxonomically by a polyphasic approach. The strains were Gram-stain-positive, coccoid, non-spore-forming and catalase-negative bacteria. Morphological, physiological and phylogenetic data indicated that these isolates belonged to the genus Lactococcus. Strain 117(T) was closely related to Lactococcus fujiensis, Lactococcus lactis subsp. lactis, L. lactis subsp. cremoris, L. lactis subsp. hordniae, L. lactis subsp. tructae and Lactococcus taiwanensis, showing 93-94% and 82-89% 16S rRNA and rpoB gene sequence similarities, respectively. Strain 4195(T) was closely related to Lactococcus chungangensis, Lactococcus raffinolactis, Lactococcus plantarum and Lactococcus piscium, showing 92-98% and 86-99% 16S rRNA and rpoB gene sequence similarities, respectively. Based on this evidence and the data obtained in the present study, the milk isolates represent two novel species of the genus Lactococcus, for which the names Lactococcushircilactis sp. nov., and Lactococcuslaudensis sp. nov. are proposed. The respective type strains are 117(T) ( = LMG 28352(T) = DSM 28960(T)) and 4195(T )( = LMG 28353(T) = DSM 28961(T)).
从意大利瓦尔泰利纳的山羊奶和意大利特龙皮亚山谷的牛奶中分别分离出两株乳酸菌，分别命名为117(T)和4195(T)，并采用多相分类法对其进行分类学特征分析。这些菌株为革兰氏阳性、球状、无芽孢形成且过氧化氢酶阴性的细菌。形态学、生理学和系统发育数据表明，这些分离株属于乳球菌属。菌株117(T)与富士乳球菌、乳酸乳球菌乳酸亚种、乳酸乳球菌乳脂亚种、乳酸乳球菌霍氏亚种、乳酸乳球菌特鲁克亚种和台湾乳球菌密切相关，16S rRNA和rpoB基因序列相似性分别为93-94%和82-89%。菌株4195(T)与忠川乳球菌、棉籽糖乳球菌、植物乳球菌和鱼乳球菌密切相关，16S rRNA和rpoB基因序列相似性分别为92-98%和86-99%。基于这些证据以及本研究获得的数据，这些牛奶分离株代表了乳球菌属的两个新物种，分别命名为山羊乳球菌新种（Lactococcushircilactis sp. nov.）和劳德乳球菌新种（Lactococcuslaudensis sp. nov.）。其相应的模式菌株分别为117(T)（= LMG 28352(T) = DSM 28960(T)）和4195(T)（= LMG 28353(T) = DSM 28961(T)）。
