白细胞介素-10启动子基因型对成人哮喘易感性的影响

The Contribution of Interleukin-10 Promoter Genotypes to Susceptibility to Asthma in Adults.

作者信息

Hsia Te-Chun, Chang Wen-Shin, Wang Shengyu, Shen Te-Chun, Hsiao Wan-Yun, Liu Chin-Jung, Liang Shinn-Jye, Chen Wei-Chun, Tu Chih-Yen, Tsai Chia-Wen, Hsu Chin-Mu, Bau Da-Tian

机构信息

Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Department of Respiratory Therapy, China Medical University, Taichung, Taiwan, R.O.C.

Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.

出版信息

In Vivo. 2015 Nov-Dec;29(6):695-9.

PMID:26546526

Abstract

AIM

Accumulating evidence suggests that inflammatory processes play a role in asthma etiology, and interleukin-10 (IL10) is an important immunosuppressive cytokine. The present study aimed to evaluate the contribution of IL10 promoter A-1082G (rs1800896), T-819C (rs3021097), A-592C (rs1800872) genetic polymorphisms to the risk of asthma in Taiwan.

MATERIALS AND METHODS

Associations of three IL10 polymorphic genotypes with risk of asthma were investigated among 198 patients with asthma and 453 non-asthmatic healthy controls, by polymerase chain reaction-restriction fragment length polymorphism genotyping method.

RESULTS

The results showed that the percentages of TT, TC and CC for IL-10 T-819C genotypes were differentially represented at 63.1%, 32.3% and 4.6%, respectively, in the patient group and 53.0%, 36.4% and 10.6%, respectively, in the healthy control group (p for trend=0.0114). The CC genotype carriers were at lower risk for asthma (odds ratio=0.36, 95% confidence interval=0.17-0.76, p=0.0055). There was no difference in the distribution of A-1082G or A-592C genotype between the asthma and non-asthma groups. The protective effects of the CC genotype were obvious among males, but not females, and those aged 25 up to 40 years but not those aged over 40 years.

CONCLUSION

The CC genotype of IL10 T-819C compared to the TT genotype may have a protective effect on asthma risk in younger adults (25-40 years old), and males in Taiwan.

摘要

目的

越来越多的证据表明炎症过程在哮喘病因中起作用，而白细胞介素-10（IL10）是一种重要的免疫抑制细胞因子。本研究旨在评估IL10启动子A-1082G（rs1800896）、T-819C（rs3021097）、A-592C（rs1800872）基因多态性对台湾地区哮喘发病风险的影响。

材料与方法

采用聚合酶链反应-限制性片段长度多态性基因分型方法，对198例哮喘患者和453例非哮喘健康对照者进行研究，分析三种IL10基因多态性基因型与哮喘发病风险的相关性。

结果

结果显示，患者组中IL-10 T-819C基因型的TT、TC和CC比例分别为63.1%、32.3%和4.6%，健康对照组分别为53.0%、36.4%和10.6%（趋势p值=0.0114）。CC基因型携带者患哮喘的风险较低（比值比=0.36，95%置信区间=0.17-0.76，p=0.0055）。哮喘组和非哮喘组之间A-1082G或A-592C基因型的分布没有差异。CC基因型的保护作用在男性中明显，但在女性中不明显；在25至40岁的人群中明显，但在40岁以上的人群中不明显。