Abiodun O O, Sood S, Osiyemi O A, Agnihotri V K, Gulati A, Ajaiyeoba E O, Singh B
Afr J Med Med Sci. 2015 Mar;44(1):21-6.
The spread of microorganisms resistant to some antimicrobial agents necessitates the need to search for novel and effective antimicrobial agents. In this study, the antimicrobial activity of Terminalid catappa Linn. (Combretaceae) and Vitex doniana Sweet. (Verbenaceae), two Nigerian medicinal plants used in folk medicines for the management of various ailments related to microbial infections were evaluated.
To evaluate the antimicrobial activity of the crude ethanol extracts and fractions of the leaves and stem bark of T. catappa and V. doniana.
Four crude ethanol extracts and 16 (n-hexane, ethylacetate, n-butanol and aqueous) fractions of leaves and stem bark of T. catappa and V doniana were evaluated for in vitro antimicrobial activity against fifteen (15) strains of bacteria and fungi. The antimicrobial activity was determined in a 96-well plate using a resazurin based broth microdilution method. Two standard antimicrobial drugs ampicillin and nystatin were included as positive control.
The butanoL fraction of stem bark of T. catappa and ethanol crude extract of leaf of V don iana displayed the highest antibacterial activity with similar minimum inhibitory concentration (MIC) value of 93.75 microg/mL against S. aureus and B. subtilis. Furthermore, the ethyl acetate fraction of stem bark of T. catappa showed the highest antifungal activity with MIC of 187.5 microg/mL against A. sydowi. Amp icillin had MIC of 15.6 and 31.3 microg/mL against S. aureus and B. subtili, respectively while nystatin produced MIC of 3.9 microg/mL against A. sydowi.
Termninalia catappa and Vitex doniana may serve as useful sources of plant derived antimicrobial agents.
对某些抗菌药物耐药的微生物的传播使得有必要寻找新型有效的抗菌药物。在本研究中，对两种尼日利亚药用植物诃子（使君子科）和钝叶牡荆（马鞭草科）的抗菌活性进行了评估，这两种植物在民间医学中用于治疗与微生物感染相关的各种疾病。
评估诃子和钝叶牡荆叶及茎皮的粗乙醇提取物及其馏分的抗菌活性。
对诃子和钝叶牡荆叶及茎皮的四种粗乙醇提取物和16种（正己烷、乙酸乙酯、正丁醇和水）馏分进行了针对15株细菌和真菌的体外抗菌活性评估。使用基于刃天青的肉汤微量稀释法在96孔板中测定抗菌活性。将两种标准抗菌药物氨苄青霉素和制霉菌素作为阳性对照。
诃子茎皮的正丁醇馏分和钝叶牡荆叶的乙醇粗提物对金黄色葡萄球菌和枯草芽孢杆菌表现出最高的抗菌活性，其最低抑菌浓度（MIC）值相似，均为93.75微克/毫升。此外，诃子茎皮的乙酸乙酯馏分对西氏曲霉表现出最高的抗真菌活性，MIC为187.5微克/毫升。氨苄青霉素对金黄色葡萄球菌和枯草芽孢杆菌的MIC分别为15.6和31.3微克/毫升，而制霉菌素对西氏曲霉的MIC为3.9微克/毫升。
诃子和钝叶牡荆可能是植物源抗菌剂的有用来源。
