Hoeller Ulrich, Baur Manuela, Roos Franz F, Brennan Lorraine, Daniel Hannelore, Fallaize Rosalind, Forster Hannah, Gibney Eileen R, Gibney Mike, Godlewska Magdalena, Hartwig Kai, Kolossa Silvia, Lambrinou Christina P, Livingstone Katherine M, Lovegrove Julie A, Macready Anna L, Manios Yannis, Marsaux Cyril F M, Martinez J Alfredo, Celis-Morales Carlos, Moschonis George, Navas-Carretero Santiago, O'Donovan Clare B, San-Cristobal Rodrigo, Saris Wim H M, Surwiłło Agnieszka, Traczyk Iwona, Tsirigoti Lydia, Walsh Marianne C, Woolhead Clara, Mathers John C, Weber Peter
1DSM Nutritional Products,Analytical Research Centre and Human Nutrition & Health,4002 Basel,Switzerland.
2UCD Institute of Food and Health,University College Dublin,Belfield,Dublin 4,Republic of Ireland.
Br J Nutr. 2016 Jan 28;115(2):202-11. doi: 10.1017/S0007114515004298. Epub 2015 Nov 9.
An efficient and robust method to measure vitamin D (25-hydroxy vitamin D3 (25(OH)D3) and 25-hydroxy vitamin D2 in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3 were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3 levels (P<0·001 for each case). Seasonal effects were modelled by fitting a sine function with a minimum 25(OH)D3 level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson's correlation between the measured values and the predicted values was r 0·65, and the sd of their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.
已开发出一种高效且稳健的方法来测量干血斑（DBS）中的维生素D（25-羟基维生素D3（25(OH)D3）和25-羟基维生素D2），并将其应用于泛欧洲多中心、基于互联网的个性化营养干预研究Food4Me。该方法包括用含有内源性25(OH)D3的血液进行校准，将其作为DBS点样并校正血细胞比容含量。该方法按照国际标准进行了验证。虽然所有参数的性能特征未达到当前血浆中液相色谱 - 质谱/质谱的金标准，但发现其非常适合在现场条件下进行状态水平测定。DBS样本质量非常高，从1465名参与者中获得了3778次25(OH)D3测量值。研究中心以及研究中心内的季节是25(OH)D3水平的良好预测指标（每种情况P<0·001）。通过拟合一个正弦函数来模拟季节效应，25(OH)D3的最低水平出现在1月20日，最高水平出现在7月21日。季节幅度因中心而异。德国冬季和夏季水平之间的差异最大，波兰最小。对该模型进行交叉验证以确定预测的一致性和DBS方法的性能。测量值与预测值之间的Pearson相关性为r 0·65，其差异的标准差为21·2 nmol/l。这包括分析变异和个体内的生物学变异。总体而言，通过参与者在家中无监督采样获得的DBS是在大型营养研究中获取维生素D状态信息的可行方法。
