Geenens Rachel, Famaey Nele, Gijbels Andy, Verhulst Valérie, Vinckier Stefan, Vander Sloten Jos, Herijgers Paul
Department of Cardiovascular Sciences, KU Leuven, Herestraat, Leuven, Belgium.
Ann Thorac Cardiovasc Surg. 2016;22(1):38-43. doi: 10.5761/atcs.oa.15-00225. Epub 2015 Nov 6.
To compensate for the lack of haptic feedback by surgical robots, limitation of exerted forces could be implemented. The limits should be based on the observed relationship between tissue load and induced damage. This study examines whether age-related changes influence this relationship.
Descending thoracic aortas of male C57BL/6J mice of 10, 25 and 40 weeks were clamped in vivo (no clamp, 0.5N or 2.0N) for 2 min. Functional integrity was tested in vitro by studying endothelium-dependent and -independent vasoreactivity.
Endothelium-dependent relaxation deteriorated with increased clamping force at all ages. Clamping did not influence endothelium-independent vasodilation. Age (10, 25 and 40 weeks) did not significantly impact on the effect of clamping on endothelium-dependent and independent vasoreactivity.
Within the tested conditions, mechanical clamping induces damage to the vascular endothelium, but not to the smooth muscle cells. Age has no effect on the obtained results in mice from 10 to 40 weeks old.
为弥补手术机器人触觉反馈的不足，可实施力施加限制。这些限制应基于观察到的组织负荷与所致损伤之间的关系。本研究探讨年龄相关变化是否会影响这种关系。
将10周、25周和40周龄雄性C57BL/6J小鼠的降主动脉在体内进行夹闭（无夹闭、0.5N或2.0N）2分钟。通过研究内皮依赖性和非内皮依赖性血管反应性在体外测试功能完整性。
在所有年龄段，内皮依赖性舒张功能均随夹闭力增加而恶化。夹闭不影响非内皮依赖性血管舒张。年龄（10周、25周和40周）对夹闭对内皮依赖性和非内皮依赖性血管反应性的影响无显著影响。
在测试条件下，机械夹闭会导致血管内皮损伤，但不会导致平滑肌细胞损伤。年龄对10至40周龄小鼠的所得结果无影响。
