Duan C X, Li W C, Zhu Z D, Li D D, Sun S L, Wang X M
Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China.
College of Life Sciences,Henan Normal University,Xinxiang 453007,China.
Bull Entomol Res. 2016 Feb;106(1):124-34. doi: 10.1017/S0007485315000863. Epub 2015 Nov 9.
Callosobruchus chinensis (Linnaeus) is one of the most destructive pests of leguminous seeds. Genetic differentiation and diversity analysis of 345 C. chinensis individuals from 23 geographic populations using 20 polymorphic simple sequence repeats revealed a total of 149 alleles with an average of 7.45 alleles per locus. The average Shannon's information index was 1.015. The gene flow and genetic differentiation rate values at the 20 loci ranged from 0.201 to 1.841 and 11.0-47.2%, with averages of 0.849 and 24.4%, respectively. In the 23 geographic populations, the effective number of alleles and observed heterozygosity ranged from 1.441 to 2.218 and 0.191-0.410, respectively. Shannon's information index ranged from 0.357 to 0.949, with the highest value in Hohhot and the lowest in Rudong. In all comparisons, the fixation index (F ST ) values ranged from 0.049 to 0.441 with a total F ST value of 0.254 among the 23 C. chinensis populations, indicating a moderate level of genetic differentiation and gene flow among these populations. Analysis of molecular variance revealed that the genetic variation within populations accounted for 76.7% of the total genetic variation. The genetic similarity values between populations varied from 0.617 to 0.969, whereas genetic distances varied from 0.032 to 0.483. Using unweighted pair-group method using arithmetical averages cluster analysis, the 23 geographic collections were classified into four distinct genetic groups but most of them were clustered into a single group. The pattern of the three concentrated groups from polymerase chain reactions analysis showed a somewhat different result with cluster.
绿豆象（Callosobruchus chinensis (Linnaeus)）是豆科种子最具破坏性的害虫之一。利用20个多态性简单序列重复对来自23个地理种群的345个绿豆象个体进行遗传分化和多样性分析，共检测到149个等位基因，每个位点平均有7.45个等位基因。平均香农信息指数为1.015。20个位点的基因流和遗传分化率值在0.201至1.841之间，遗传分化率在11.0%至47.2%之间，平均值分别为0.849和24.4%。在23个地理种群中，有效等位基因数和观察杂合度分别在1.441至2.218和0.191至0.410之间。香农信息指数在0.357至0.949之间，呼和浩特地区最高，如东地区最低。在所有比较中，固定指数（F ST）值在0.049至0.441之间，23个绿豆象种群的总F ST值为0.254，表明这些种群之间存在中等程度的遗传分化和基因流。分子方差分析表明，种群内的遗传变异占总遗传变异的76.7%。种群间的遗传相似性值在0.617至0.969之间，而遗传距离在0.032至0.483之间。采用算术平均数非加权配对组法聚类分析，23个地理样本被分为四个不同的遗传组，但大多数样本聚为一组。聚合酶链反应分析的三个集中组模式显示出与聚类结果略有不同的结果。
