Bharti Shreekant, Dogra Sunil, Saikia Biman, Walker Ranjana Minz, Chhabra Seema, Saikia Uma Nahar
Department of Immunopathology, PGIMER, Chandigarh, India.
Indian J Pathol Microbiol. 2015 Oct-Dec;58(4):479-82. doi: 10.4103/0377-4929.168850.
The direct immunofluorescence (DIF) of skin in conjunction with histopathology gives the best diagnostic yield. It is invaluable in confirming the diagnosis of small vessel vasculitides and bullous lesions of the skin and can be used as an additional tool to pinpoint the diagnosis of systemic and localized autoimmune diseases involving the skin. This study was undertaken to analyze the strength of DIF vis-à -vis histopathology in the diagnosis of discoid lupus erythematosus (DLE) and at the same time to elaborate the specific immunofluorescence findings in the lesions of DLE. The clinical profile and cutaneous lesions of 75 patients with DLE are described. DIF was positive in 68% and histopathology in 60% of cases. The most common immunoreactant was IgG at the dermoepidermal junction, followed by IgM and IgA. A conclusive diagnosis of DLE could be achieved satisfactorily in 64 cases (85%) by a combination of the two techniques.
皮肤直接免疫荧光检查（DIF）结合组织病理学检查，诊断阳性率最高。在确诊皮肤小血管炎和大疱性病变方面，它具有重要价值，还可作为辅助手段，精准诊断累及皮肤的全身性和局限性自身免疫性疾病。本研究旨在分析DIF相对于组织病理学检查在盘状红斑狼疮（DLE）诊断中的优势，同时阐述DLE皮损的特异性免疫荧光表现。描述了75例DLE患者的临床特征和皮肤损害情况。DIF检查阳性率为68%，组织病理学检查阳性率为60%。最常见的免疫反应物是位于真皮表皮交界处的IgG，其次是IgM和IgA。两种技术联合应用，64例（85%）患者可获得满意的DLE确诊结果。
