嗜热自养甲烷杆菌δH菌株中一个与氢化酶相关的基因编码一种多铁氧化还原蛋白。
A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin.
作者信息
Reeve J N, Beckler G S, Cram D S, Hamilton P T, Brown J W, Krzycki J A, Kolodziej A F, Alex L, Orme-Johnson W H, Walsh C T
机构信息
Department of Microbiology, Ohio State University, Columbus 43210.
出版信息
Proc Natl Acad Sci U S A. 1989 May;86(9):3031-5. doi: 10.1073/pnas.86.9.3031.
Abstract
The genes mvhDGA, which encode the subunit polypeptides of the methyl viologen-reducing hydrogenase in Methanobacterium thermoautotrophicum strain delta H, have been cloned and sequenced. These genes, together with a fourth open reading frame designated mvhB, are tightly linked and appear to form an operon that is transcribed starting 42 base pairs upstream of mvhD. The organization and sequences of the mvhG and mvhA genes indicate a common evolutionary ancestry with genes encoding the small and large subunits of hydrogenases in eubacterial species. The product of the mvhB gene is predicted to contain six tandomly repeated bacterial-ferredoxin-like domains and, therefore, is predicted to be a polyferredoxin that could contain as many as 48 iron atoms in 12 Fe4S4 clusters.
摘要
编码嗜热自养甲烷杆菌δH菌株中甲基紫精还原氢化酶亚基多肽的基因mvhDGA已被克隆和测序。这些基因与第四个开放阅读框mvhB紧密相连，似乎形成了一个操纵子，该操纵子从mvhD上游42个碱基对处开始转录。mvhG和mvhA基因的组织和序列表明，它们与真细菌物种中编码氢化酶小亚基和大亚基的基因有着共同的进化起源。预测mvhB基因的产物含有六个串联重复的细菌铁氧化还原蛋白样结构域，因此预计是一种多铁氧化还原蛋白，在12个Fe4S4簇中可能含有多达48个铁原子。
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