Lee Hyojung, Lee Hyo Jin, Kim Ji Yeon, Kwon Oran
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 139-743, Korea.
Mol Cells. 2015 Dec;38(12):1044-53. doi: 10.14348/molcells.2015.0107. Epub 2015 Nov 6.
This study first investigated the effects of corn gluten hydrolysate (CGH) (1.5 g/day) administration for 7 days on appetite-responsive genes in lean Sprague-Dawley (SD) rats. In a second set of experiments, the metabolic changes occurring at multiple time points over 8 weeks in response to CGH (35.33% wt/wt) were observed in high-fat (HF, 60% of energy as fat) diet-fed SD rats. In lean rats, the hypothalamus neuropeptide-Y and proopiomelanocortin mRNA levels of the CGH group were significantly changed in response to CGH administration. In the second part of the study, CGH treatment was found to reduce body weight and perirenal and epididymal fat weight. CGH also prevented an increase in food intake at 2 weeks and lowered plasma leptin and insulin levels in comparison with the HF group. This reduction in the plasma and hepatic lipid levels was followed by improved insulin resistance, and the beneficial metabolic effects of CGH were also partly related to increases in plasma adiponectin levels. The Homeostasis Model of Assessment - Insulin Resistance (HOMA-IR), an index of insulin resistance, was markedly improved in the HF-CGH group compared with the HF group at 6 weeks. According to the microarray results, adipose tissue mRNA expression related to G-protein coupled receptor protein signaling pathway and sensory perception was significantly improved after 8 weeks of CGH administration. In conclusion, the present findings suggest that dietary CGH may be effective for improving hyperglycemia, dyslipidemia and insulin resistance in diet-induced obese rats as well as appetite control in lean rats.
本研究首先调查了连续7天给予玉米麸质水解物（CGH）（1.5克/天）对瘦型斯普拉格-道利（SD）大鼠食欲响应基因的影响。在第二组实验中，观察了高脂（HF，60%的能量来自脂肪）饮食喂养的SD大鼠在8周内多个时间点对CGH（35.33%重量/重量）的代谢变化。在瘦型大鼠中，CGH组的下丘脑神经肽Y和阿黑皮素原mRNA水平在给予CGH后发生了显著变化。在研究的第二部分中，发现CGH治疗可减轻体重以及肾周和附睾脂肪重量。与HF组相比，CGH还在2周时阻止了食物摄入量的增加，并降低了血浆瘦素和胰岛素水平。血浆和肝脏脂质水平的这种降低伴随着胰岛素抵抗的改善，并且CGH的有益代谢作用还部分与血浆脂联素水平的增加有关。在6周时，与HF组相比，HF-CGH组的胰岛素抵抗指数——稳态模型评估-胰岛素抵抗（HOMA-IR）明显改善。根据微阵列结果，给予CGH 8周后，与G蛋白偶联受体蛋白信号通路和感觉知觉相关的脂肪组织mRNA表达显著改善。总之，目前的研究结果表明，饮食中的CGH可能对改善饮食诱导的肥胖大鼠的高血糖、血脂异常和胰岛素抵抗以及瘦型大鼠的食欲控制有效。
