LKB1在胃癌中的临床意义及作用

Clinical significance and role of LKB1 in gastric cancer.

作者信息

Jiang Shanshan, Chen Rensheng, Yu Jianan, Li Nianshuang, Ke Rong, Luo Lingyu, Zou Junrong, Zhang Jiangnan, Zhang Kunhe, Lu Nonghua, Huang Deqiang

机构信息

Research Institute of Digestive Diseases, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

出版信息

Mol Med Rep. 2016 Jan;13(1):249-56. doi: 10.3892/mmr.2015.4508. Epub 2015 Nov 5.

DOI:10.3892/mmr.2015.4508

PMID:26549522

Abstract

Liver kinase B1 (LKB1) functions as a tumor suppressor gene, and loss in the expression of LKB1 contributes to human carcinogenesis and tumor progression. The present study investigated the association between LKB1 and gastric cancer. SGC‑7901 gastric cancer cell lines and 63 patients with gastric cancer were examined in the present study, and lentivirus transfection, reverse transription‑quantitative polymerase chain reaction and flow cytometric analyses were performed. By examining the expression of LKB1 using immunohistochemical analyses, the present study found that the expression of LKB1 was reduced in the gastric cancer tissues, and restoration of the expression of LKB1 reduced tumor cell viability, migration rate and the expression of CD44, induced cell cycle arrest at the G2 phase of the cell cycle, and increased the sensitivity of the gastric cancer cells to anticancer drugs. LKB1 protein is a tumor‑suppressor in gastric cancer and may be potentially be developed as a novel gene therapy target in the treatment of gastric cancer.

摘要

肝脏激酶B1（LKB1）作为一种肿瘤抑制基因发挥作用，LKB1表达缺失会促进人类癌症发生和肿瘤进展。本研究调查了LKB1与胃癌之间的关联。本研究检测了SGC-7901胃癌细胞系和63例胃癌患者，并进行了慢病毒转染、逆转录-定量聚合酶链反应和流式细胞术分析。通过免疫组化分析检测LKB1的表达，本研究发现LKB1在胃癌组织中的表达降低，LKB1表达的恢复降低了肿瘤细胞活力、迁移率和CD44的表达，诱导细胞周期停滞于细胞周期的G2期，并增加了胃癌细胞对抗癌药物的敏感性。LKB1蛋白是胃癌中的一种肿瘤抑制因子，可能有潜力被开发为治疗胃癌的新型基因治疗靶点。