Jiang Jiong, Dong Lei, Shi Hai-Tao, Guo Xiao-Yan, Qin Bin, Wang Yan, Li Hong
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710003, P.R. China.
Mol Med Rep. 2016 Jan;13(1):393-7. doi: 10.3892/mmr.2015.4524. Epub 2015 Nov 6.
Imiquimod, the most prominent Toll‑like receptor 7 agonist, has direct anti‑tumor activity and can induce autophagy and apoptosis in various types of human cancer. The aim of the present study was to examine the anti‑tumor effects of imiquimod and their underlying mechanisms in SGC‑7901 cells. Imiquimod exerted an inhibitory effect on cell proliferation in a dose‑ and time‑dependent manner as indicated by an MTT assay. Imiquimod induced autophagy as well as apoptosis, while simultaneous treatment with 3‑methyladenine (3-MA), an autophagy inhibitor, decreased the toxicity of imiquimod. Furthermore, blocking of autophagy by 3‑MA exerted an inhibitory effect on imiquimod-induced apoptosis, which indicated that autophagy can function as a mechanism which, upon activation, directly leads to apoptosis and cell death of SGC‑7901 cells. The results of the present study suggested that imiquimod has potent direct activity against gastric cancer cells by inducing autophagy and apoptosis.
咪喹莫特是最著名的Toll样受体7激动剂，具有直接抗肿瘤活性，可诱导多种类型人类癌症发生自噬和凋亡。本研究旨在探讨咪喹莫特对SGC-7901细胞的抗肿瘤作用及其潜在机制。MTT试验表明，咪喹莫特对细胞增殖具有剂量和时间依赖性抑制作用。咪喹莫特可诱导自噬以及凋亡，而同时用自噬抑制剂3-甲基腺嘌呤（3-MA）处理可降低咪喹莫特的毒性。此外，3-MA阻断自噬对咪喹莫特诱导的凋亡具有抑制作用，这表明自噬可作为一种机制，激活后直接导致SGC-7901细胞凋亡和死亡。本研究结果提示，咪喹莫特通过诱导自噬和凋亡对胃癌细胞具有强大的直接活性。
