School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
Sichuan Key Laboratory of Medical American Cockroach, Chengdu, Sichuan 610000, P.R. China.
Mol Med Rep. 2017 Dec;16(6):9043-9050. doi: 10.3892/mmr.2017.7716. Epub 2017 Oct 4.
Gastric cancer is a leading cause of cancer‑associated mortality worldwide. In studies on the mechanisms of antigastric cancer drugs, autophagy and endoplasmic reticulum (ER) stress have been demonstrated to serve an active role in gastric cancer. The organic extract of Periplaneta americana (also termed American Cockroach), which is named Kangfuxin (KFX) in China, has been used clinically as a traditional Chinese medicine against disorders, including stomach bleeding, gastric ulcers, tuberculosis, burns and trauma. However, the role of KFX and its mechanism in gastric cancer remains to be elucidated. The present study aimed to determine the effects of KFX in vitro against cultured the human carcinoma SGC‑7901 cell line, and to explore the potential mechanism of the anticancer effects of KFX in gastric cancer. SGC‑7901 cells were treated with different concentrations of KFX for varying amounts of time. As a result, KFX treatment decreased the ratio of apoptosis regulators Bcl‑2/Bax, activated ER stress and induced significant apoptosis in SGC‑7901 cells. Furthermore, KFX was able to restore the ER stress activation blocked by 4‑phenylbutyrate. In addition, KFX activated autophagy in SGC‑7901 cells. These results demonstrated that ER stress, autophagy and the apoptosis‑inducing effects of KFX in SGC‑7901 cells may achieve promising anticancer effects in numerous other types of cancer. In particular, ER stress may serve an essential role in KFX‑induced anticancer effects on gastric carcinoma and a secondary role in autophagy.
胃癌是全球癌症相关死亡的主要原因。在研究抗胃癌药物的机制时，自噬和内质网（ER）应激被证明在胃癌中发挥着积极的作用。美洲大蠊（也称为美洲大蠊）的有机提取物，在中国被命名为康复新液（KFX），已被临床用作治疗包括胃出血、胃溃疡、肺结核、烧伤和创伤等疾病的传统中药。然而，KFX 在胃癌中的作用及其机制仍有待阐明。本研究旨在确定 KFX 在体外对人癌细胞 SGC-7901 细胞系的作用，并探讨 KFX 在胃癌中抗癌作用的潜在机制。SGC-7901 细胞用不同浓度的 KFX 处理不同的时间。结果表明，KFX 处理降低了凋亡调节因子 Bcl-2/Bax 的比值，激活了 ER 应激，并诱导 SGC-7901 细胞发生明显的细胞凋亡。此外，KFX 能够恢复 4-苯基丁酸阻断的 ER 应激激活。此外，KFX 在 SGC-7901 细胞中激活了自噬。这些结果表明，ER 应激、自噬和 KFX 在 SGC-7901 细胞中诱导凋亡的作用可能在其他许多类型的癌症中产生有希望的抗癌效果。特别是，ER 应激可能在 KFX 诱导的胃癌抗癌作用中发挥重要作用，在自噬中发挥次要作用。
