Sadeghi Soha, Sanati Mohammad Hossein, Taghizadeh Morteza, Mansouri Parvine, Jadali Zohreh
Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology , Tehran , Iran.
Razi Vaccine and Serum Research Institute , Karaj , Iran.
Acta Microbiol Immunol Hung. 2015 Sep;62(3):275-85. doi: 10.1556/030.62.2015.3.5.
Alopecia areata represents an autoimmune pathological process driven primarily by cellular aberrations contained within the immune system, which activates various humoral and cellular elements of the immune response. The aim of this study was to determine the mRNA expression levels of T-bet and GATA-3 as potential inducers of T helper (Th)1 and Th2 differentiation, respectively, as well as Th1(IFN-γ) and Th2(IL-4) cytokine mRNA expression in patients with alopecia areata. Using real-time reverse transcriptase PCR (RT-PCR), the relative amounts of T-bet, GATA-3, IFN-γ, and IL-4 mRNA transcripts were determined in PBMCs from 20 Iranian patients with alopecia areata and compared with those of 20 healthy control subjects. In comparison with the normal group, T-bet and IFN-γ mRNA expression levels were significantly up-regulated in the alopecia areata patients, while GATA-3 and IL-4 mRNA expression levels were down-regulated. Notably, positive correlation (P < 0.05) was found between IFN-γ and T-bet levels in patients and controls. In addition, significant positive correlations existed between GATA-3 and IL-4 (P < 0.05). These results indicate that a Th1/Th2 imbalance exists in alopecia areata, and it may be implicated in the pathogenesis of disease.
斑秃是一种主要由免疫系统内细胞异常驱动的自身免疫病理过程，该过程会激活免疫反应的各种体液和细胞成分。本研究的目的是确定T-bet和GATA-3的mRNA表达水平，它们分别是T辅助细胞（Th）1和Th2分化的潜在诱导因子，同时确定斑秃患者中Th1（IFN-γ）和Th2（IL-4）细胞因子的mRNA表达。使用实时逆转录聚合酶链反应（RT-PCR），测定了20名伊朗斑秃患者外周血单个核细胞（PBMCs）中T-bet、GATA-3、IFN-γ和IL-4 mRNA转录本的相对含量，并与20名健康对照者的进行比较。与正常组相比，斑秃患者中T-bet和IFN-γ mRNA表达水平显著上调，而GATA-3和IL-4 mRNA表达水平下调。值得注意的是，在患者和对照者中，IFN-γ和T-bet水平之间存在正相关（P < 0.05）。此外，GATA-3和IL-4之间存在显著正相关（P < 0.05）。这些结果表明斑秃中存在Th1/Th2失衡，这可能与疾病的发病机制有关。
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