Chen Li, Li Tao, Wang Yan-li, Dong Yu, Zhuang Shuai-xing, Wang Dun-fang, Yang Wei-peng
Yao Xue Xue Bao. 2015 Jul;50(7):887-92.
A simple and selective HPLC method for simultaneous determination and quantification of anthraquinones, lignans and flavonoids in Xiao-Cheng-Qi Tang (XCQT), Hou-Po-San-Wu Tang (HPSWT) and Hou-Po-Da-Huang Tang (HPDHT) was developed and validated. An Agilent Zorbax SB-C 18 (4.6 mm x 250 mm, 5 µm) column with the mobile phase of acetonitrile and 0.5% acetic acid aqueous solution in gradient elution mode was used. The flow rate was 1.0 mL · min(-1) at 30 °C, and injection volume was 10 µL. The detection wavelength was set at 254 nm and 294 nm simultaneously for the quantitative analysis. The current HPLC assay was validated for linearity, intra-day and inter-day precisions, accuracy, recovery and stability. The method was applied to the content comparison of the gallic acid, cinnamic acid, sennoside A, sennoside B, rhein, emodin, aloe-emodin, chrysophanol, physcion, magnolol, honokiol, narirutin, naringin, hesperidin, neohesperidin, hesperetin, naringenin and nobiletin in XCQT, HPSWT and HPDHT. The good linear equations of eighteen constituents were obtained within the investigated ranges (r > 0.998). The recovery of the method was 94.28%-99.89% and the precision was less than 5%. The sample was stable within 16 h. There were some differences between the contents of anthraquinones, lignans and flavonoids in analogous formulae about XCQT. XCQT contained the greatest abundance of anthraquinones and flavonoid, HPSWT contained the greatest abundance lignans. In conclusion, the methods are simple, low-cost, precise, accurate and reliable for the determination of eighteen constituents in analogous formulae about XCQT, and these results provide methodological support for its quality control.
建立并验证了一种简单且具选择性的高效液相色谱法，用于同时测定和定量小承气汤（XCQT）、厚朴三物汤（HPSWT）和厚朴大黄汤（HPDHT）中的蒽醌类、木脂素类和黄酮类成分。采用安捷伦Zorbax SB-C 18柱（4.6 mm×250 mm，5 µm），以乙腈和0.5%乙酸水溶液为流动相，采用梯度洗脱模式。流速为1.0 mL·min⁻¹，柱温30℃，进样量为10 µL。同时设定检测波长为254 nm和294 nm进行定量分析。对当前的高效液相色谱法进行了线性、日内和日间精密度、准确度、回收率及稳定性验证。该方法应用于XCQT、HPSWT和HPDHT中没食子酸、肉桂酸、番泻苷A、番泻苷B、大黄酸、大黄素、芦荟大黄素、 Chrysophanol、 大黄酚、厚朴酚、和厚朴酚、橙皮芸香苷、柚皮苷、橙皮苷、新橙皮苷、橙皮素、柚皮素和诺米林的含量比较。在所研究的范围内，18种成分均获得了良好的线性方程（r＞0.998）。该方法的回收率为94.28% - 99.89%，精密度小于5%。样品在16小时内稳定。XCQT类似方剂中蒽醌类、木脂素类和黄酮类成分的含量存在一些差异。XCQT中蒽醌类和黄酮类成分含量最高，HPSWT中木脂素类成分含量最高。总之，该方法简单、低成本、精确、准确且可靠，可用于测定XCQT类似方剂中的18种成分，这些结果为其质量控制提供了方法学支持。
