[高效液相色谱法同时测定六味安消胶囊中蒽醌类成分]

[Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC].

作者信息

Jiang Bo, Gao Xun, Bi Kai-Shun, Chen Xiao-Hui

机构信息

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Zhong Yao Cai. 2009 Jun;32(6):975-7.

PMID:19764339

Abstract

OBJECTIVE

To establish an RP-HPLC method for measuring the contents of aloe-emodin, rhein, emodin, chrysophanol and physion in Liuwei'anxiao Capsules (traditional Chinese medicine).

METHODS

The analysis was carried out with a Diamonsil-C18 column (200 mm x 4.6 mm, 5 microm) and a mobile phase of methanol-0.05% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min and the column temperature was 35 degrees C. The detection wavelength was 254 nm.

RESULTS

The calibration curve was linear within the range of 1.622 - 16.22 microg/mL (r1 = 0.9998), 0.6960 - 6.960 microg/mL (r2 = 0.9998), 2.448 - 24.48 microg/mL (r3 = 0.9995), 2.752 - 27.52 microg/mL (r4 = 0.9997), 2.702 -27.02 microg/mL (r = 0.9997) for aloe-emodin, rhein, emodin, chrysophanol and physion, respectively.

CONCLUSION

This method is convenient, accurate and reproducible for the quality control of Liuwei'anxiao Capsules.

摘要

目的

建立测定六味安消胶囊（中药）中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的反相高效液相色谱法。

方法

采用Diamonsil-C18柱（200 mm×4.6 mm，5μm），以甲醇-0.05%磷酸溶液为流动相进行梯度洗脱。流速为1.0 mL/min，柱温为35℃，检测波长为254 nm。

结果

芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的校准曲线在1.622 - 16.22μg/mL（r1 = 0.9998）、0.6960 - 6.960μg/mL（r2 = 0.9998）、2.448 - 24.48μg/mL（r3 = 0.9995）、2.752 - 27.52μg/mL（r4 = 0.9997）、2.702 - 27.02μg/mL（r = 0.9997）范围内呈线性关系。