矽肺大鼠肺组织中水通道蛋白-1表达降低。

Decreased Expression of Aquaporin-1 in Lung Tissue of Silicotic Rats.

作者信息

Wang Hongli, Hao Xiaohui, Zhang Li, Liu Shupeng, Wang Qian, Liu Nan, Zhao Jinyuan, Q Jiang, Liu Heliang

出版信息

Clin Lab. 2015;61(9):1163-9. doi: 10.7754/clin.lab.2015.150223.

DOI:10.7754/clin.lab.2015.150223

PMID:26554235

Abstract

BACKGROUND

Aquaporin-1 (AQP-1), found in the early 1990s, a water channel protein in the cell membranes of mammals, has been reported to play an important role in water balance of the respiratory system. However, there are a few studies about the role of AQP in occupational pulmonary disease such as silicosis. This study is to explore the information of aquaporin-1 (AQP-1) in the pathogenesis of silicosis by examining AQP expression, distribution, and location in the lung tissue of a silicotic rat model.

METHODS

Male Wistar SPF rats were divided randomly into the following 8 groups (n = 8 per group): (1) saline control group: instillation of 1 mL sterile physiological saline; (2) silica groups (ld, 7d, 14d, 28d, 42d, 56d): instillation of a suspension of 50 mg silica dust in a total volume of 1 mL sterile physiological saline; (3) the normal control group without treatment. Immunohistochemistry, immunofluorescence, and western blot were used to detect distribution and expression of AQP-1 in the lung tissue of rats exposed to silica.

RESULTS

The expression of AQP-1 between normal and the saline control rats showed no significant difference, but was decreased in the silicotic model rats' lung.

CONCLUSIONS

The expression of AQP-1 decreased in silicotic rats, which suggests that AQP-1 may play an important role in the formation of silicosis.

摘要

背景

水通道蛋白-1（AQP-1）于20世纪90年代初被发现，是哺乳动物细胞膜中的一种水通道蛋白，据报道在呼吸系统的水平衡中起重要作用。然而，关于水通道蛋白在职业性肺病如矽肺中的作用的研究较少。本研究旨在通过检测水通道蛋白-1（AQP-1）在矽肺大鼠模型肺组织中的表达、分布和定位，探讨其在矽肺发病机制中的作用。

方法

将雄性Wistar SPF大鼠随机分为以下8组（每组n = 8）：（1）生理盐水对照组：滴注1 mL无菌生理盐水；（2）二氧化硅组（1天、7天、14天、28天、42天、56天）：滴注50 mg二氧化硅粉尘悬浮液，总体积为1 mL无菌生理盐水；（3）未处理的正常对照组。采用免疫组织化学、免疫荧光和蛋白质印迹法检测二氧化硅暴露大鼠肺组织中AQP-1的分布和表达。