Matsumura Y, Ikegawa R, Ohyama T, Hayashi K, Morimoto S
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Biochem Biophys Res Commun. 1989 Apr 28;160(2):602-8. doi: 10.1016/0006-291x(89)92475-3.
Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250-350 pg/10(6) cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3-24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells.
利用内皮素（ET）的放射免疫测定法（RIA），我们测定了猪主动脉内皮细胞培养基中的免疫反应性ET（IR-ET）。将培养基中的免疫反应性与生物活性进行了比较。释放到培养基中的IR-ET量计算为250 - 350 pg/10(6) 细胞/小时。随着孵育3 - 24小时，释放到培养基中的IR-ET量逐渐增加，并且与培养基诱导的大鼠离体主动脉血管收缩增加相对应。当将培养基中的血管收缩活性与通过RIA测定的IR-ET浓度作图时，浓度-反应曲线显示出与用合成猪ET获得的曲线相似。这种RIA系统将有助于研究内皮细胞分泌ET的机制。
