Rieder H, Ramadori G, Meyer zum Büschenfelde K H
I. Medizinische Klinik und Poliklinik, Johannes-Gutenberg Universität, Mainz.
Klin Wochenschr. 1991 Jun 18;69(9):387-91. doi: 10.1007/BF01647411.
Endothelin is the most potent vasoconstrictor peptide known today. Using a radioimmunoassay for endothelin, we measured immunoreactive endothelin in culture media of guinea pig sinusoidal endothelial liver cells and human umbilical vein endothelial cells. A time-dependent release of immunoreactive endothelin by confluent sinusoidal endothelial liver cells in culture was found. Sinusoidal endothelial liver cells produced similar amounts of immunoreactive endothelin as umbilical vein endothelial cells, about 900 pg/microgram DNA per 24 h. In the presence of transforming growth factor beta a dose-dependent increase of immunoreactive endothelin release was measured. The maximal increase of 50% was found at a concentration of 1 ng transforming growth factor per ml. To a similar extent Kupffer cell-conditioned media augmented the release of immunoreactive endothelin by sinusoidal endothelial liver cells, especially when Kupffer cells had been stimulated by endotoxin. Endotoxin itself did not alter the release of immunoreactive endothelin. Endothelin released by sinusoidal endothelial liver cells might influence the pericytes of the liver, i.e., the Ito-cells.
内皮素是目前已知最强效的血管收缩肽。我们使用内皮素放射免疫分析法，检测了豚鼠肝血窦内皮细胞和人脐静脉内皮细胞培养基中的免疫反应性内皮素。结果发现，培养的汇合肝血窦内皮细胞能随时间依赖性释放免疫反应性内皮素。肝血窦内皮细胞产生的免疫反应性内皮素量与人脐静脉内皮细胞相似，每24小时约为900 pg/μg DNA。在转化生长因子β存在的情况下，可检测到免疫反应性内皮素释放呈剂量依赖性增加。在每毫升1 ng转化生长因子的浓度下，最大增加量为50%。库普弗细胞条件培养基在相似程度上增强了肝血窦内皮细胞免疫反应性内皮素的释放，尤其是当库普弗细胞受到内毒素刺激时。内毒素本身并不改变免疫反应性内皮素的释放。肝血窦内皮细胞释放的内皮素可能会影响肝脏的周细胞，即贮脂细胞。
