Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.
Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
ACS Appl Mater Interfaces. 2015 Dec 2;7(47):26124-30. doi: 10.1021/acsami.5b07692. Epub 2015 Nov 20.
This work reports the assembly of thin films of a silica (SiO2)-modified copper-metal organic framework, Cu3(BTC)2 [Cu3(BTC)2@SiO2, BTC = benzene-1,3,5-tricarboxylic acid] on a conducting substrate of NH2-BDC [NH2-BDC = 2-aminobenzene-1,4-dicarboxylic acid] doped polyaniline (PANI). Assembled Cu3(BTC)2@SiO2/BDC-PANI thin films displayed electrical conductivity in the range of 35 μA. These thin films were conjugated with antiatrazine antibodies to create a novel immunosensing platform. Various structural and spectral characteristics of the synthesized material and its bioconjugate were investigated. The developed immunosensor was used for the conductometric sensing of atrazine. The detection of atrazine was achieved with a high sensor sensitivity (limit of detection = 0.01 nM) and specificity in the presence of diverse pesticides (e.g., endosulfan, parathion, paraoxon, malathion, and monochrotophos).
这项工作报道了将硅（SiO2）改性的铜金属有机骨架，Cu3（BTC）2[Cu3（BTC）2@SiO2，BTC=苯-1,3,5-三羧酸]组装到掺杂 NH2-BDC[NH2-BDC=2-氨基苯-1,4-二羧酸]的导电基底上的过程。组装的 Cu3（BTC）2@SiO2/BDC-PANI 薄膜的电导率在 35 μA 范围内。这些薄膜与抗阿特拉津抗体结合，形成了一种新型的免疫传感平台。对合成材料及其生物缀合物的各种结构和光谱特性进行了研究。所开发的免疫传感器用于阿特拉津的电导传感。在存在多种农药（如内硫磷、对硫磷、对氧磷、马拉硫磷和氧乐果）的情况下，该传感器对阿特拉津的检测具有高传感器灵敏度（检测限=0.01 nM）和特异性。
